Notch-1 Antibody (N1A) [Phycoerythrin]

RPMI 8226 human multiple myeloma cell line was stained with Mouse Anti-Mouse Notch-1 PE-conjugated Monoclonal Antibody (Catalog # IC5267P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Product Discontinued

Notch-1 Antibody (N1A) [Phycoerythrin] Summary

• Immunogen: Mouse embryonic fibroblast cell line PA317-derived recombinant mouse Notch-1
  Accession # Q01705
• Specificity: Detects mouse Notch-1 intracellular domain in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NOTCH1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Notch-1 Antibody (N1A) [Phycoerythrin]

• EC 2.1.2.11
• EC 3.4.21.68
• hN1
• Notch (Drosophila) homolog 1 (translocation-associated)
• notch 1Notch homolog 1, translocation-associated (Drosophila)
• Notch homolog 1, translocation-associated
• Notch1
• Notch-1
• TAN1
• TAN1neurogenic locus notch homolog protein 1
• Translocation-associated notch protein TAN-1

Background

Notch-1 is a 300 kDa type I transmembrane glycoprotein that is one of four Notch homologues involved in developmental processes (1-3). Notch signaling is important for maintaining stem cells and inducing differentiation, especially in the nervous system and lymphoid tissues (2-4). Notch can specify binary cell fates. For example, it promotes T cell over B cell development from a common precursor (2). Mouse Notch-1 is synthesized as a 2531 amino acid (aa) precursor that contains an 18 aa signal sequence, a 1707 aa extracellular domain (ECD) with 36 EGF-like repeats and three Lin-12/notch repeats (LNR), a 21 aa transmembrane (TM) segment and a 785 aa cytoplasmic domain that contains six ankyrin repeats, a glutamine-rich domain and a PEST sequence. The 11^th and 12^th EGF-like repeats, that bind ligands such as Jagged and Delta-like families in humans, correspond to aa 412-488 in mouse Notch-1 (6). Elongation of O-linked fucose chains by Fringe family members at a site within this region can inhibit the interaction of Notch with Jagged ligands, thereby promoting Delta-like ligand interactions (7). The Notch-1 receptor undergoes post-translational furin-type proteolytic cleavage, generating a heterodimer through the interaction of a hydrophobic area C-terminal to the LNR on the extracellular region with the transmembrane/cytoplasmic portion (8, 9). Upon ligand binding, additional sequential proteolysis by TNF-converting enzyme (ADAM17) and the presenilin-dependent gamma -secretase results in the release of the Notch intracellular domain (NICD) which translocates into the nucleus, activating transcription of Notch-responsive genes (10).

1. Ellisen, L.W. et al. (1991) Cell 66:649.
2. Dumortier, A. et al. (2005) Int. J. Hematol. 82:277.
3. Yoon, K. and N. Gaiano (2005) Nat. Neurosci. 8:709.
4. Androutsellis-Theotokis, A. et al. (2006) Nature 442:823.
5. Weng, A.P. et al. (2004) Science 306:269.
6. Hambleton, S. et al. (2004) Structure 12:2173.
7. Yang, L. et al. (2005) Mol. Biol. Cell 16:927.
8. Sanchez-Irizarry, C. et al. (2004) Mol. Cell. Biol. 24:9265.
9. Logeat, F. et al. (1998) Proc. Natl. Acad. Sci. USA 95:8108.
10. Mumm, J.S. and R. Kopan (2000) Dev. Biol. 228:151.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Notch-1 Antibody (IC5267P). (Showing 1 - of FAQ).

Bioinformatics

• Gene Symbol: NOTCH1
• Uniprot:
  • Q01705()