Nope/IGDCC4 Antibody [Biotin]

• Reactivity: Mu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Nope/IGDCC4 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Nope/IGDCC4
  Gly22-His953
  Accession # Q9EQS9
• Specificity: Detects mouse Nope/IGDCC4 in ELISAs and Western blots. In sandwich immunoassays, less than 0.3% cross-reactivity with recombinant mouse (rm) DCC, rmNeogenin, and rmPUNC is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IGDCC4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse Nope Antibody (Catalog # AF1394)
  ELISA Detection: Mouse Nope Biotinylated Antibody (Catalog # BAF1394)
  Standard: Recombinant Mouse Nope Fc Chimera (Catalog # 1394-NP)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nope/IGDCC4 Antibody [Biotin]

• DDM36
• hDDM36
• IGDCC4
• immunoglobulin superfamily DCC subclass member 4
• immunoglobulin superfamily, DCC subclass, member 4
• KIAA1628
• likely ortholog of mouse neighbor of Punc E11
• LOC57722
• Neighbor of punc e11
• Nope
• NOPEFLJ42051
• Protein DDM36

Background

Mouse Nope (Neighbor Of Punc E11) was discovered as a gene proximal to the Punc gene on chromosome 9 (1). Punc and Nope are distant members of a subgroup of the immunoglobulin (Ig) superfamily, which include DCC (Deleted in Colorectal Cancer) (2), Caenorhabditis elegans UNC5 (UNC = behaviorally uncoordinated) and its mammalian homologues (rat UNC5H1 and H2, mouse UNC5H2 and H3, and human UNC5H3 and H4) (3), Drosophila Frazzled (4), vertebrate Neogenin (5), and mouse Nope and mouse Punc. Members of this subgroup of the Ig superfamily are type I transmembrane proteins with four Ig domains in their extracellular regions.

Mouse Nope consists of a 21 amino acid (aa) signal peptide, a 933 aa extracellular domain (including four Ig domains, five fibronectin-type III (FnIII) repeats), a 24 aa transmembrane segment, and a 274 aa cytoplasmic domain (1). The extracellular domain of mouse Nope shares 45% aa sequence similarity with mouse Punc. However, the cytoplasmic domains of mouse Nope and mouse Punc do not share aa sequence similarity. Compared to other members of the subgroup of the Ig superfamily, mouse Nope extracellular domain shares 25.8% and 25.2% aa similarity with mouse DCC and mouse Neogenin, respectively. Mouse and human Nope share 90.8% aa sequence similarity. Mouse Nope is expressed mostly in embryonic muscle tissues and in developing and adult nervous systems. The structural similarities between Nope and the guidance receptor of the DCC family suggest that Nope may have similar functions as the DCC family (6‑8).

1. Salbaum, J.M. and C. Kappen (2000) Genomics 64:15.
2. Fearon et al. (1990) Science 247:49.
3. Keino-Masu et al. (1996) Cell 87:175.
4. Hong, K. et al. (1999) Cell 97:927.
5. Leonardo, E.D. et al. (1997) Nature 386:833.
6. Kolodziej et al. (1996) Cell 87:197.
7. Meyerhardt (1997) Oncogene 14:1129.
8. Vielmetter et al. (1997) Genomics 41:414.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGDCC4
• Uniprot:
  • Q9EQS9()