Protogenin Antibody (OTI2B3)

Western Blot: Protogenin Antibody (2B3) [NBP2-02745] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Protogenin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Protogenin.

Immunocytochemistry/Immunofluorescence: Protogenin Antibody (OTI2B3) [NBP2-02745] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PRTG.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: OTI2B3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Protogenin Antibody (OTI2B3) Summary

• Description: Novus Biologicals Mouse Protogenin Antibody (OTI2B3) (NBP2-02745) is a monoclonal antibody validated for use in WB and ICC/IF. Anti-Protogenin Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human PRTG (NP_776175) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRTG
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:2000
• Theoretical MW: 126.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Protogenin Antibody (OTI2B3)

• FLJ12795
• FLJ13221
• FLJ25756
• IGDCC5
• immunoglobulin superfamily, DCC subclass, member 5
• Protein Shen-Dan
• protogenin homolog (Gallus gallus)
• Protogenin
• PRTG
• RPTG
• Shen-Dan

Background

PRTG may play a role in anteroposterior axis elongation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Protogenin Antibody (NBP2-02745)(1)

Publication using NBP2-02745

• Visvanathan A, Saulnier O, Chen C et Al. Early rhombic lip Protogenin(+ve) stem cells in a human-specific neurovascular niche initiate and maintain group 3 medulloblastoma Cell 2024-07-05 [PMID: 38971152]

Bioinformatics

• Gene Symbol: PRTG
• Entrez:
  • 283659(Human)