ACRBP Antibody

Images

 
Western Blot: ACRBP Antibody [NBP1-85407] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: ACRBP Antibody [NBP1-85407] - Staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.
Orthogonal Strategies: Immunohistochemistry-Paraffin: ACRBP Antibody [NBP1-85407] - Staining in human testis and endometrium tissues using anti-ACRBP antibody. Corresponding ACRBP RNA-seq data are presented for ...read more
Immunohistochemistry-Paraffin: ACRBP Antibody [NBP1-85407] - Staining of human endometrium shows low expression as expected.
Immunohistochemistry-Paraffin: ACRBP Antibody [NBP1-85407] - Staining of human testis shows high expression.

Product Details

Summary
Reactivity Hu, Pm, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

ACRBP Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: AKRVLCSQPVSILSPNTLKEIEASAEVSPTTMTSPISPHFTVTERQTFQPWPERLSNNVEELLQSSLSLGGQEQAPEHKQEQGVEHRQEPTQEHKQE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACRBP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:5000 - 1:10000
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
  • Western Blot 0.04-0.4 ug/ml
Theoretical MW
61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
ACRBP Protein (NBP1-85407PEP)
Publications
Read Publications using
NBP1-85407 in the following applications:

Reactivity Notes

Monkey reactivity reported in the literature (PMID: 30358843). Use in Rhesus Macaque reported in secitific publication PMID: 32351003

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ACRBP Antibody

  • acrosin binding protein
  • acrosin-binding protein
  • Cancer/testis antigen 23
  • Cancer/testis antigen OY-TES-1
  • CT23FLJ51160
  • OY-TES-1
  • proacrosin binding protein sp32
  • Proacrosin-binding protein sp32
  • SP32

Background

ACRBP is encoded by this gene is similar to proacrosin binding protein sp32 precursor found in mouse, guinea pig, and pig. This protein is located in the sperm acrosome and is thought to function as a binding protein to proacrosin for packaging and condensation of the acrosin zymogen in the acrosomal matrix. This protein is a member of the cancer/testis family of antigens and it is found to be immunogenic. In normal tissues, this mRNA is expressed only in testis, whereas it is detected in a range of different tumor types such as bladder, breast, lung, liver, and colon. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ACRBP Antibody (NBP1-85407)(2)

We have publications tested in 2 confirmed species: Monkey, Rhesus Macaque.

We have publications tested in 2 applications: ICC/IF, IHC-P.


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ICC/IF
(1)
IHC-P
(1)
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Monkey
(1)
Rhesus Macaque
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ACRBP
Entrez
Uniprot