NOP56 Antibody - BSA Free

Western Blot: NOP56 Antibody [NBP2-13666] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-NOP56 antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.

Immunocytochemistry/Immunofluorescence: NOP56 Antibody [NBP2-13666] - Staining of human cell line U-2 OS shows localization to nucleoli fibrillar center. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: NOP56 Antibody [NBP2-13666] - Human kidney shows weak positivity in nucleoli in cells in tubules.

Immunohistochemistry-Paraffin: NOP56 Antibody [NBP2-13666] - Human pancreas shows weak postivity in nucleoli in exocrine glandular cells.

Immunohistochemistry-Paraffin: NOP56 Antibody [NBP2-13666] - Staining of human fallopian tube shows moderate postivity in nucleoli in glandular cells.

Immunohistochemistry-Paraffin: NOP56 Antibody [NBP2-13666] - Staining of human cerebral cortex shows strong positivity in nucleoli in neurons.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NOP56 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NOP56 Antibody - BSA Free (NBP2-13666) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: EDPSISFSKPKKKKSFSKEELMSSDLEETAGSTS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NOP56
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  NOP56 Recombinant Protein Antigen (NBP2-13666PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for NOP56 Antibody - BSA Free

• NOL5A
• NOP56 ribonucleoprotein homolog (yeast)
• nucleolar protein 56
• nucleolar protein 5A (56kD with KKE/D repeat)
• nucleolar protein 5A (56kDa with KKE/D repeat)
• Nucleolar protein 5A

Background

Nop56p is a yeast nucleolar protein that is part of a complex with the nucleolar proteins Nop58p and fibrillarin. Nop56p is required for assembly of the 60S ribosomal subunit and is involved in pre-rRNA processing. The protein encoded by this gene is similar in sequence to Nop56p and is also found in the nucleolus. Multiple transcript variants encoding several different isoforms have been found for this gene, but the full-length nature of most of them have not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NOP56
• Entrez:
  • 10528(Human)