NOP56 Antibody

Western Blot: NOP56 Antibody [NBP1-46847] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NOP56 antibody NBP1-46847 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. NOP56 was also immunoprecipitated by rabbit anti-NOP56 antibody NBP1-46848, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Immunohistochemistry-Paraffin: NOP56 Antibody [NBP1-46847] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-NOP56 used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

NOP56 Antibody Summary

• Description: Novus Biologicals Rabbit NOP56 Antibody (NBP1-46847) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-NOP56 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this product maps to a region between residue 494 and 544 of human NOP56 ribonucleoprotein homolog using the numbering given in entry NP_006383.2 (GeneID 10528).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NOP56
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Theoretical MW: 66 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NOP56 Antibody

• NOL5A
• NOP56 ribonucleoprotein homolog (yeast)
• nucleolar protein 56
• nucleolar protein 5A (56kD with KKE/D repeat)
• nucleolar protein 5A (56kDa with KKE/D repeat)
• Nucleolar protein 5A

Background

Nop56p is a yeast nucleolar protein that is part of a complex with the nucleolar proteins Nop58p and fibrillarin. Nop56p is required for assembly of the 60S ribosomal subunit and is involved in pre-rRNA processing. The protein encoded by this gene is similar in sequence to Nop56p and is also found in the nucleolus. Multiple transcript variants encoding several different isoforms have been found for this gene, but the full-length nature of most of them have not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NOP56 Antibody (NBP1-46847)(2)

Publications using NBP1-46847

• Wang X, Li ZT, Yan Y et al. LARP7-Mediated U6 snRNA Modification Ensures Splicing Fidelity and Spermatogenesis in Mice Mol. Cell 2020-01-20 [PMID: 32017896]
• Chauvin C, Koka V, Nouschi A et al. Ribosomal protein S6 kinase activity controls the ribosome biogenesis transcriptional program. Oncogene 2014-01-23 [PMID: 23318442] (WB, Mouse)

Bioinformatics

• Gene Symbol: NOP56
• Entrez:
  • 10528(Human)
• Uniprot:
  • O00567(Human)