NOP56 Antibody (CL2603) - Azide and BSA Free

Staining of human breast cancer shows strong nucleolar positivity in tumor cells.

Staining in HeLa cell line with Anti-NOP56 monoclonal antibody) .

Staining in A431 cell line with Anti-NOP56 monoclonal antibody) .

Staining in U251 cell line with Anti-NOP56 monoclonal antibody) .

Staining in U2OS cell line with Anti-NOP56 monoclonal antibody) .

Staining in MCF7 cell line with Anti-NOP56 monoclonal antibody) .

Lane 1: Marker [kDa]Lane 2:Human cell line U-251 MG

Staining of human fallopian tube shows strong nucleolar immunoreactivity in glandular and connective tissue cells.

Staining of human kidney shows strong nucleolar positivity in renal tubules.

Staining of human cervix shows strong nucleolar immunoreactivity in the epithelial cells.

Staining of human prostate cancer shows strong nucleolar positivity in glandular cells.

Staining of human liver shows strong nucleolar positivity in hepatocytes.

Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-NOP56 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL2603
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

NOP56 Antibody (CL2603) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse NOP56 Antibody (CL2603) - Azide and BSA Free (NBP2-36778) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of O00567, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NOP56
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for NOP56 Antibody (CL2603) - Azide and BSA Free

• NOL5A
• NOP56 ribonucleoprotein homolog (yeast)
• nucleolar protein 56
• nucleolar protein 5A (56kD with KKE/D repeat)
• nucleolar protein 5A (56kDa with KKE/D repeat)
• Nucleolar protein 5A

Background

Nop56p is a yeast nucleolar protein that is part of a complex with the nucleolar proteins Nop58p and fibrillarin. Nop56p is required for assembly of the 60S ribosomal subunit and is involved in pre-rRNA processing. The protein encoded by this gene is similar in sequence to Nop56p and is also found in the nucleolus. Multiple transcript variants encoding several different isoforms have been found for this gene, but the full-length nature of most of them have not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NOP56