Nogo Receptor/NgR Antibody

Images

 
Western Blot: Nogo Receptor/NgR Antibody [NB100-1536] - (0.3ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Nogo Receptor/NgR Antibody Summary

Immunogen
Peptide with sequence C-LQALPDDTFRDLGN corresponding to internal region according to NP_075380.1.
Epitope
internal region
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RTN4R
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Experiments gave bands at approx. 90-100 kDa band and 37+40+45 kDa band in human brain lysate after 0.3ug/ml antibody staining. These bands were successfully blocked by the immunizing peptide and they correspond to earlier findings in scientific literature with different antibodies (Wang et al, J Neurosci. 2002 Jul 1;22(13): 5505-15). This protein has a calculated MW of 50.7 kDa band according to NP_075380.1.
Positive Control
Nogo Receptor/NgR Lysate (NBL1-15614)
Brain Lysate (NB820-59177)
Publications
Read Publication using NB100-1536.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Porcine, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Nogo Receptor/NgR Antibody

  • NgR
  • NgR1
  • Nogo R
  • Nogo receptor
  • Nogo-66 receptor
  • NogoR
  • NOGORUNQ330/PRO526
  • reticulon 4 receptor
  • reticulon-4 receptor
  • RTN4R

Background

This gene encodes the receptor for reticulon 4, oligodendrocyte myelin glycoprotein and myelin-associated glycoprotein. This receptor mediates axonal growth inhibition and may play a role in regulating axonal regeneration and plasticity in the adult central nervous system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nogo Receptor/NgR Antibody (NB100-1536)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RTN4R
Entrez
Uniprot