Nogo Receptor/NgR Antibody [Unconjugated]

• Reactivity: Hu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Nogo Receptor/NgR Antibody [Unconjugated] Summary

• Immunogen: recombinant human NgR
  Cys27-Ser447
  Accession # Q9BZR6
• Specificity: Detects human Nogo Receptor (NgR) in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RTN4R
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nogo Receptor/NgR Antibody [Unconjugated]

• NgR
• NgR1
• Nogo R
• Nogo receptor
• Nogo-66 receptor
• NogoR
• NOGORUNQ330/PRO526
• reticulon 4 receptor
• reticulon-4 receptor
• RTN4R

Background

Nogo Receptor (NgR), also named reticulon 4 receptor, is a glycosylphosphoinositol (GPI)-anchored protein that belongs to the family of leucine-rich repeat (LRR) proteins (1). It is expressed predominantly in the central nervous systems in neurons and their axons. NgR plays an essential role in mediating axon growth inhibition induced by the structurally distinct myelin-derived proteins Nogo, myelin-associated glycoprotein (MAG), and myelin oligodendrocyte glycoprotein (Omgp) (2, 3). Human NgR cDNA encodes a 473 amino acid residues (aa) precursor with a 26 aa putative signal peptide, an LRR-type N-terminal region, eight LRR repeats, a cysteine-rich LRR-type C-terminal region, a GPI linkage domain and a 26 aa C-terminal propeptide that is removed in the mature form (1). All of the LRR domains within NgR are required for ligand binding and receptor oligomerization (4). NgR mediates its inhibitory actions by interacting with the p75 neurotrophin receptor (p75^NTR), a tumor necrosis factor receptor superfamily (TNFRSF) member also known for modulating the activities of the Trk family of receptor tyrosine kinases, and for inducing apoptosis in neurons and oligodendrocytes (5). Upon ligand binding, NgR binds to and activates the p75^NTR. The activated p75^NTR then sequesters the Rho guanine dissociation inhibitor (Rho-GDI) away from Rho and allows Rho to change into the active GTP-bound state which can interact with signaling proteins to suppress axonal growth and regeneration (4). The truncated extracellular domain of NgR has been shown to bind the myelin-derived inhibitors and block inhibition of axon growth by myelin (6).

1. Fournier, A.E. et al. (2001) Nature 409:341.
2. GrandPre, T. et al. (2002) Nature 417:547.
3. Wang, K.C. et al. (2002) Nature 420:74.
4. Barton, W.A. et al. (2003) EMBO Journal 22:3291.
5. Yamashita, T. and M. Tohyama (2003) Nature Neuroscience 6:461.
6. Fournier, A.S. et al. (2002) J. Neurosci. 22:8876.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nogo Receptor/NgR Antibody (AF1208)(8)

Publications using AF1208

• D Ben Amar, K Thoinet, B Villalard, O Imbaud, C Costechare, L Jarrosson, F Reynaud, J Novion Duc, Y Couté, JF Brunet, V Combaret, N Corradini, C Delloye-Bo, V Castellani Environmental cues from neural crest derivatives act as metastatic triggers in an embryonic neuroblastoma model Nature Communications, 2022-05-10;13(1):2549. 2022-05-10 [PMID: 35538114] (Neutralization, Human)
• Y Sekine, PT Algarate, WBJ Cafferty, SM Strittmatt PlexinA2 and CRMP2 Signaling Complex is Activated by Nogo-A-Liganded NgR1 to Restrict Corticospinal Axon Sprouting after Trauma J. Neurosci., 2019-02-25;0(0):. 2019-02-25 [PMID: 30804090] (ICC)
• Kurihara Y, Iketani M, Ito H, Nishiyama K, Sakakibara Y, Goshima Y, Takei K LOTUS suppresses axon growth inhibition by blocking interaction between Nogo receptor-1 and all four types of its ligand. Mol Cell Neurosci, 2014-07-15;61(0):211-8. 2014-07-15 [PMID: 25034269] (ICC, Primate - Chlorocebus pygerythrus (Vervet Monkey))
• Jennifer L. Konopka-Anstadt, Bernardo A. Mainou, Danica M. Sutherland, Yuichi Sekine, Stephen M. Strittmatter, Terence S. Dermody The Nogo Receptor NgR1 Mediates Infection by Mammalian Reovirus Cell Host & Microbe 2014-06-11 [PMID: 24922571]
• Heather D. VanGuilder Starkey, William E. Sonntag, Willard M. Freeman Increased hippocampal NgR1 signaling machinery in aged rats with deficits of spatial cognition European Journal of Neuroscience 2013-05-01 [PMID: 23438185]
• Kurihara Y, Arie Y, Iketani M, Ito H, Nishiyama K, Sato Y, Nakamura F, Mizuki N, Goshima Y, Takei K The carboxyl-terminal region of Crtac1B/LOTUS acts as a functional domain in endogenous antagonism to Nogo receptor-1. Biochem. Biophys. Res. Commun., 2012-01-18;418(2):390-5. 2012-01-18 [PMID: 22281491] (ICC, Mouse)
• Yan J, Zhou X, Guo JJ, Mao L, Wang YJ, Sun J, Sun LX, Zhang LY, Zhou XF, Liao H Nogo-66 inhibits adhesion and migration of microglia via GTPase Rho pathway in vitro. J. Neurochem., 2012-01-23;120(5):721-31. 2012-01-23 [PMID: 22145612] (ELISA Development, ICC, Western Blot, Rat)
• Williams G, Wood A, Williams EJ, Gao Y, Mercado ML, Katz A, Joseph-McCarthy D, Bates B, Ling HP, Aulabaugh A, Zaccardi J, Xie Y, Pangalos MN, Walsh FS, Doherty P Ganglioside inhibition of neurite outgrowth requires Nogo receptor function: identification of interaction sites and development of novel antagonists. J. Biol. Chem., 2008-04-14;283(24):16641-52. 2008-04-14 [PMID: 18411262] (Immunoprecipitation, Western Blot, Human)

Reviews for Nogo Receptor/NgR Antibody (AF1208) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Mouse.

reviewed by: Verified Customer IF Human 01/05/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 22281491
• Species: Human

reviewed by: Verified Customer ICC Mouse 01/05/2015

Summary

• Application: Immunocytochemistry
• Sample Tested: See PMID 22281491
• Species: Mouse

Bioinformatics

• Gene Symbol: RTN4R
• Uniprot:
  • Q9BZR6()