Nogo Receptor/NgR Antibody [Unconjugated]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Nogo Receptor/NgR Antibody [Unconjugated] Summary

Immunogen
recombinant human NgR
Cys27-Ser447
Accession # Q9BZR6
Specificity
Detects human Nogo Receptor (NgR) in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant mouse NgR is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RTN4R
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Blockade of Receptor-ligand Interaction
Reviewed Applications
Read 2 Reviews rated 4
using
AF1208 in the following applications:

Publications
Read Publications using
AF1208 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nogo Receptor/NgR Antibody [Unconjugated]

  • NgR
  • NgR1
  • Nogo R
  • Nogo receptor
  • Nogo-66 receptor
  • NogoR
  • NOGORUNQ330/PRO526
  • reticulon 4 receptor
  • reticulon-4 receptor
  • RTN4R

Background

Nogo Receptor (NgR), also named reticulon 4 receptor, is a glycosylphosphoinositol (GPI)-anchored protein that belongs to the family of leucine-rich repeat (LRR) proteins (1). It is expressed predominantly in the central nervous systems in neurons and their axons. NgR plays an essential role in mediating axon growth inhibition induced by the structurally distinct myelin-derived proteins Nogo, myelin-associated glycoprotein (MAG), and myelin oligodendrocyte glycoprotein (Omgp) (2, 3). Human NgR cDNA encodes a 473 amino acid residues (aa) precursor with a 26 aa putative signal peptide, an LRR-type N-terminal region, eight LRR repeats, a cysteine-rich LRR-type C-terminal region, a GPI linkage domain and a 26 aa C-terminal propeptide that is removed in the mature form (1). All of the LRR domains within NgR are required for ligand binding and receptor oligomerization (4). NgR mediates its inhibitory actions by interacting with the p75 neurotrophin receptor (p75NTR), a tumor necrosis factor receptor superfamily (TNFRSF) member also known for modulating the activities of the Trk family of receptor tyrosine kinases, and for inducing apoptosis in neurons and oligodendrocytes (5). Upon ligand binding, NgR binds to and activates the p75NTR. The activated p75NTR then sequesters the Rho guanine dissociation inhibitor (Rho-GDI) away from Rho and allows Rho to change into the active GTP-bound state which can interact with signaling proteins to suppress axonal growth and regeneration (4). The truncated extracellular domain of NgR has been shown to bind the myelin-derived inhibitors and block inhibition of axon growth by myelin (6).

  1. Fournier, A.E. et al. (2001) Nature 409:341.
  2. GrandPre, T. et al. (2002) Nature 417:547.
  3. Wang, K.C. et al. (2002) Nature 420:74.
  4. Barton, W.A. et al. (2003) EMBO Journal 22:3291.
  5. Yamashita, T. and M. Tohyama (2003) Nature Neuroscience 6:461.
  6. Fournier, A.S. et al. (2002) J. Neurosci. 22:8876.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nogo Receptor/NgR Antibody (AF1208)(5)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Primate - Chlorocebus pygerythrus (Vervet Monkey).

We have publications tested in 3 applications: ICC, Immunoprecipitation, Western Blot.


Filter By Application
ICC
(3)
Immunoprecipitation
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
Rat
(1)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
(1)
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Showing Publications 1 - 5 of 5.
Publications using AF1208 Applications Species
Y Sekine, PT Algarate, WBJ Cafferty, SM Strittmatt PlexinA2 and CRMP2 Signaling Complex is Activated by Nogo-A-Liganded NgR1 to Restrict Corticospinal Axon Sprouting after Trauma J. Neurosci., 2019;0(0):. 2019 [PMID: 30804090] (ICC) ICC
Kurihara Y, Iketani M, Ito H, Nishiyama K, Sakakibara Y, Goshima Y, Takei K LOTUS suppresses axon growth inhibition by blocking interaction between Nogo receptor-1 and all four types of its ligand. Mol Cell Neurosci, 2014;61(0):211-8. 2014 [PMID: 25034269] (ICC, Primate - Chlorocebus pygerythrus (Vervet Monkey)) ICC Primate - Chlorocebus pygerythrus (Vervet Monkey)
Kurihara Y, Arie Y, Iketani M, Ito H, Nishiyama K, Sato Y, Nakamura F, Mizuki N, Goshima Y, Takei K The carboxyl-terminal region of Crtac1B/LOTUS acts as a functional domain in endogenous antagonism to Nogo receptor-1. Biochem. Biophys. Res. Commun., 2012;418(2):390-5. 2012 [PMID: 22281491] (ICC, Mouse) ICC Mouse
Yan J, Zhou X, Guo JJ, Mao L, Wang YJ, Sun J, Sun LX, Zhang LY, Zhou XF, Liao H Nogo-66 inhibits adhesion and migration of microglia via GTPase Rho pathway in vitro. J. Neurochem., 2012;120(5):721-31. 2012 [PMID: 22145612] (Western Blot, Rat) Western Blot Rat
Williams G, Wood A, Williams EJ, Gao Y, Mercado ML, Katz A, Joseph-McCarthy D, Bates B, Ling HP, Aulabaugh A, Zaccardi J, Xie Y, Pangalos MN, Walsh FS, Doherty P Ganglioside inhibition of neurite outgrowth requires Nogo receptor function: identification of interaction sites and development of novel antagonists. J. Biol. Chem., 2008;283(24):16641-52. 2008 [PMID: 18411262] (Immunoprecipitation, Human) Immunoprecipitation Human

Reviews for Nogo Receptor/NgR Antibody (AF1208) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using AF1208:
Filter by Applications
IF
(1)
ICC
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IF Human 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22281491
SpeciesHuman

Blocking

Blocking DetailsSee PMID 22281491

Details

Detection NotesSee PMID 22281491

Comments

CommentsThis antibody can be used to detect mouse Nogo receptor-1 overexpressed in COS7 cells.PMID 22281491
 
reviewed by:
Anonymous
ICC Mouse 01/05/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 22281491
SpeciesMouse

Blocking

Blocking DetailsSee PMID 22281491

Details

Detection NotesSee PMID 22281491

Comments

CommentsSee PMID 22281491

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF1208

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

 
Anonymous
01/05/2015
Application: IF
Species: Human

 
Anonymous
01/05/2015
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol RTN4R
Uniprot