Nogo Receptor/NgR Antibody (M5) [CoraFluor™ 1]

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, Flow, IP, CyTOF-ready
• Clone: M5
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: CoraFluor 1

Nogo Receptor/NgR Antibody (M5) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: (His)6-tagged fragment of rat NgR1 (amino acids 278-473).
• Specificity: The antibody binds to human and rat NgR1 with a Kd of 2.9 nM for both species (bivalent binding).
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RTN4R
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA
  • Flow Cytometry
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Nogo Receptor/NgR Antibody (M5) [CoraFluor™ 1]

• NgR
• NgR1
• Nogo R
• Nogo receptor
• Nogo-66 receptor
• NogoR
• NOGORUNQ330/PRO526
• reticulon 4 receptor
• reticulon-4 receptor
• RTN4R

Background

Nogo Receptor encodes the receptor for reticulon 4, oligodendrocyte myelin glycoprotein and myelin-associated glycoprotein. This receptor mediates axonal growth inhibition and may play a role in regulating axonal regeneration and plasticity in the adult central nervous system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RTN4R