NMDAR2B Antibody (S59) - BSA Free

Western Blot: NMDAR2B Antibody (S59) [NBP1-47611] - Western Blot analysis of Rat brain membrane lysate showing detection of GluN2B/NMDAR2B protein using Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody, Clone S59-36 (NBP1-47611). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody (NBP1-47611) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence: NMDAR2B Antibody (S59) [NBP1-47611] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody, Clone S59-36 (NBP1-47611). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody (NBP1-47611) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GluN2B/NMDAR2B Antibody (D) Composite.

Immunohistochemistry: NMDAR2B Antibody (S59) [NBP1-47611] - Immunohistochemistry analysis using Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody, Clone S59-36 (NBP1-47611). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody (NBP1-47611) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filaggrin-like staining, and dermal staining.

Western Blot: NMDAR2B Antibody (S59) [NBP1-47611] - analysis of Human HEK-T lysates showing detection of GluN2B/NR2B protein using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36 . Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:250.

Immunocytochemistry/Immunofluorescence: NMDAR2B Antibody (S59) [NBP1-47611] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Everything positive.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP, MA
• Clone: S59
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

NMDAR2B Antibody (S59) - BSA Free Summary

• Immunogen: Fusion protein amino acids 20-271 (extracellular N-terminus) of rat NR2B
• Localization: Cell Membrane , Cell Junction , Synapse , Postsynaptic Cell Membrane
• Specificity: Detects approx 166kDa. No cross-reactivity against NR2A.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: GRIN2B
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 0.1-1.0 ug/ml
  • Immunoprecipitation
  • Microarray
  • Western Blot 1:1000
• Application Notes: 1 ug/ml of GluN2B/NR2B Antibody was sufficient for detection of NR2B in 10 ug of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for NMDAR2B Antibody (S59) - BSA Free

• glutamate [NMDA] receptor subunit epsilon-2
• glutamate receptor subunit epsilon-2
• glutamate receptor, ionotropic, N-methyl D-aspartate 2B
• hNR3
• MGC142180
• NMDAR2BMGC142178
• N-methyl D-aspartate receptor subtype 2B
• N-methyl-D-aspartate receptor subunit 3
• NR2B
• NR3

Background

N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C), and NMDAR2D (GRIN2D). GRIN2B may be a candidate gene for the neurodegenerative disorder dentato-rubro-pallidoluysian atrophy (DRPLA)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRIN2B
• Entrez:
  • 2904(Human)
  • 24410(Rat)
• Uniprot:
  • Q00960(Rat)