NMDAR1 Antibody

Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody [NB100-41105] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody [NB100-41105] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

• Reactivity: Hu, Mu, Rt, Ca
• Applications: ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NMDAR1 Antibody Summary

• Description: Novus Biologicals Goat NMDAR1 Antibody (NB100-41105) is a polyclonal antibody validated for use in ICC/IF. Anti-NMDAR1 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-TQERVNNSNKKE corresponding to internal region according to NP_067544.1, NP_015566.1.
• Marker: Neuronal Marker
• Specificity: This antibody is expected to recognize all three reported isoforms (NP_000823.4; NP_067544.1; NP_015566.1).
• Predicted Species: Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GRIN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
• Theoretical MW: 105 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  NMDAR1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NMDAR1 Antibody

• glutamate [NMDA] receptor subunit zeta-1
• glutamate receptor, ionotropic, N-methyl D-aspartate 1
• NMDA1
• NMDAR1glutamate [NMDA] receptor subunit zeta 1
• NMD-R1
• N-methyl-D-aspartate receptor channel, subunit zeta-1
• N-methyl-D-aspartate receptor subunit NR1
• NR1

Background

The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. The gene consists of 21 exons and is alternatively spliced, producing transcript variants differing in the C-terminus. Although the sequence of exon 5 is identical in human and rat, the alternative exon 5 splicing in rat has yet to be demonstrated in human. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NMDAR1 Antibody (NB100-41105)(4)

Publications using NB100-41105

• Martínez-Lázaro R, Minguez-Viñas T, Reyes-Carrión A et al. GRIN2B disease-associated mutations disrupt the function of BK channels and NMDA receptor signaling nanodomains The Journal of General Physiology 2025-09-01 [PMID: 40763259]
• G�mez R, Maglio LE, Gonzalez-Hernandez AJ et al. NMDA receptor-BK channel coupling regulates synaptic plasticity in the barrel cortex Proceedings of the National Academy of Sciences 2021-08-31 [PMID: 34453004]
• Gonzalez-Gonzalez IM, Henley JMet al. Postsynaptic Kainate Receptor Recycling and Surface Expression Are Regulated by Metabotropic Autoreceptor Signalling. Traffic 2013-04-04 [PMID: 23556457] (ICC/IF, Rat)
• Rajji T, Chapman D, Eichenbaum H, Greene R. The role of CA3 hippocampal NMDA receptors in paired associate learning. J Neurosci 2006-01-18 [PMID: 16421310]

Bioinformatics

• Gene Symbol: GRIN1
• Entrez:
  • 2902(Human)
  • 14810(Mouse)
  • 24408(Rat)
• Uniprot:
  • Q05586(Human)