Western blot of rat hippocampal lysate showing specific immunolabeling of the approximately 180 kDa NR2B subunit of the NMDAR phosphorylated at Y1252 (Control). The phosphospecificity of this labeling is shown in the ...read more
NMDA (N-Methyl D-Aspartate) receptors are members of the glutamate receptor family of ligand-gated ion channels. The functional NMDA receptor (NMDAR) is a 650-850 kDa heteromultimer of at least two NR2 (NMDAR2) subunits and two NR1 subunits. NR2 subunits determine overall NMDAR characteristics and mediate NMDAR clustering and synaptic localization through cytoplasmic interaction with PSD-95/SAP90 family members. Upon glutamate binding to NR2, and glycine binding to NR1, the NMDA channel is opened, allowing calcium and sodium influx into the cell. There are four genes that code for NR2 subunits (NR2A-D). The NR2B subunit generates a high conductance NMDAR. Human NR2B (also NR3;GRIN2B and GluR epsilon 2) is a 180-200 kDa, 1458 amino acid (aa), three transmembrane (TM) glycoprotein that contains a 531 aa N-terminal extracellular domain (ECD), and a 646 aa cytoplasmic region. At least three tyrosines are known to be phosphorylated by Fyn in the cytoplasmic region. These include Y1252, Y1336 and Y1472. While much is known about Y1472, little information is available about the consequences of Y1252 and Y1336 phosphorylation.
Stephenson, F.A. (2001) Curr. Drug Targets 2:233.
Cull-candy, S.G. and D.N. Leszkiewicz (2004) Sci. STKE re16 (2004).
Prybylowski, K. and R.J. Wenthold (2004) J. Biol. Chem. 279:9673.
Ishii, T. et al. (1993) J. Biol. Chem. 268:2836.
Hess, S.D. et al. (1996) J. Pharmacol. Exp. Ther. 278:808.
Nakazawa, T. et al. (2001) J. Biol. Chem. 276:693.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our NMDA R, NR2B Subunit [p Tyr1252] Antibody [Unconjugated] and receive a gift card or discount.