NMDA R, NR2B Subunit [p Tyr1252] Antibody [Unconjugated]

Western blot of rat hippocampal lysate showing specific immunolabeling of the approximately 180 kDa NR2B subunit of the NMDAR phosphorylated at Y1252 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda PPase). The blot is identical to the control except that it was incubated in lambda PPase (1200 units for 30 minutes) before being exposed to the Anti-Phospho-NMDA NR2B subunit (Y1252). The immunolabeling is completely eliminated by treatment with lambda PPase.

• Reactivity: Hu, Rt, Pm
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

NMDA R, NR2B Subunit [p Tyr1252] Antibody [Unconjugated] Summary

• Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Y1252 of NMDA R, NR2B Subunit
• Modification: p Tyr1252
• Specificity: Human, non-human primates, and rat ~180 kDa NMDA Receptor NR2B subunit phosphorylated at Y1252 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GRIN2B
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000 dilution

Packaging, Storage & Formulations

• Storage: For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
• Buffer: 100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
• Preservative: No Preservative

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NMDA R, NR2B Subunit [p Tyr1252] Antibody [Unconjugated]

• DEE101;GluN1;Glutamate receptor;Glutamate receptor ionotropic, NMDA 1;GRIN1;MRD8;NDHMSD;NDHMSR;NMDA1;NMDAR1;NMD-R1;NR1
• GRIN2B
• NMDA R, NR2B Subunit
• NMDAR2B
• NR2B

Background

NMDA (N-Methyl D-Aspartate) receptors are members of the glutamate receptor family of ligand-gated ion channels. The functional NMDA receptor (NMDAR) is a 650-850 kDa heteromultimer of at least two NR2 (NMDAR2) subunits and two NR1 subunits. NR2 subunits determine overall NMDAR characteristics and mediate NMDAR clustering and synaptic localization through cytoplasmic interaction with PSD-95/SAP90 family members. Upon glutamate binding to NR2, and glycine binding to NR1, the NMDA channel is opened, allowing calcium and sodium influx into the cell. There are four genes that code for NR2 subunits (NR2A-D). The NR2B subunit generates a high conductance NMDAR. Human NR2B (also NR3;GRIN2B and GluR epsilon 2) is a 180-200 kDa, 1458 amino acid (aa), three transmembrane (TM) glycoprotein that contains a 531 aa N-terminal extracellular domain (ECD), and a 646 aa cytoplasmic region. At least three tyrosines are known to be phosphorylated by Fyn in the cytoplasmic region. These include Y1252, Y1336 and Y1472. While much is known about Y1472, little information is available about the consequences of Y1252 and Y1336 phosphorylation.

1. Stephenson, F.A. (2001) Curr. Drug Targets 2:233.
2. Cull-candy, S.G. and D.N. Leszkiewicz (2004) Sci. STKE re16 (2004).
3. Prybylowski, K. and R.J. Wenthold (2004) J. Biol. Chem. 279:9673.
4. Ishii, T. et al. (1993) J. Biol. Chem. 268:2836.
5. Hess, S.D. et al. (1996) J. Pharmacol. Exp. Ther. 278:808.
6. Nakazawa, T. et al. (2001) J. Biol. Chem. 276:693.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NMDA R, NR2B Subunit Antibody (PPS056)(1)

Publication using PPS056

• Chernova T, Steinert JR, Richards P Early failure of N-methyl-D-aspartate receptors and deficient spine formation induced by reduction of regulatory heme in neurons. Mol. Pharmacol., 2011-02-15;79(5):844-54. 2011-02-15 [PMID: 21325018] (ICC, Western Blot, Mouse)

Bioinformatics

• Gene Symbol: GRIN2B