NMDA R, NR1 Subunit Antibody - Prime Splice Variant - Azide and BSA Free

Western Blot: NMDAR2C Prime Splice Variant Antibody [NB300-117] - Western blot of 10 ug of HEK 293 cells expression. (Lane A) NR1-subunit that does not contain the NR1 Insert (Mock). (Lane B) NR1-subunit containing only the C2 Insert. (Lane C) NR1-subunit containing the C1 and C2 insert. (Lane D) NR1-subunit containing the C11/C2' and N1/C2' insert.

• Reactivity: Mu, Rt, Hu, Bv
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

NMDA R, NR1 Subunit Antibody - Prime Splice Variant - Azide and BSA Free Summary

• Description: The antigen is a peptide from the NR1 subunit, C2' splice variant insert of rat NMDA receptor. Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).
• Immunogen: Synthetic peptide corresponding to amino acid residues specific to the NR1 subunit, C2' splice variant insert conjugated to KLH. Accession # P35439
• Specificity: Specific for endogenous levels of the ~120 kDa NR1 subunit of the NMDA receptor containing the C2' splice variant insert. Does not recognize the NR1 subunits of the NMDA receptor that do not contain the C2' insert.
• Predicted Species: Human (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GRIN1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000 - 1:2000
  • Immunohistochemistry-Frozen 1:1000 - 1:2000
  • Western Blot 1:1000
• Theoretical MW: 120 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

It is anticipated that the antibody will also work with bovine and human tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Antigen Affinity-purified
• Reconstitution Instructions: Reconstitute with 50 uL PBS to desired concentration.

Alternate Names for NMDA R, NR1 Subunit Antibody - Prime Splice Variant - Azide and BSA Free

• GluN1
• GRIN1
• MRD8
• NDHMSD
• NDHMSR;NMD-R1
• NMDA R, NR1 Subunit
• NMDA1
• NMDAR1
• NMD-R1
• NR1

Background

The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). Increased membrane surface expression of the NMDAR, NR1-Subunit has been associated with synaptic plasticity (Grosshans et al., 2002). There are a number of different splice variants of the NR1-Subunit (Foldes et al., 1994; Zukin and Bennett, 1995). Differential splicing of three exons in the NR1-Subunit generates up to eight NR1-Subunit splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2 prime). Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRIN1
• Uniprot:
  • P35439(Rat)