NMDA R, NR1 Subunit Antibody - Splice Variant

Western Blot: NMDAR2C Splice Variant Antibody [NB300-116] - Western blot of 10 ug of HEK 293 cells expression. (Lane A) NR1-subunit that does not contain the NR1 Insert (Mock). (Lane B) NR1-subunit containing only the C2 Insert. (Lane C) NR1-subunit containing the C1 and C2 in sert. (Lane D) NR1-subunit containing the C1/C2' and N1/C2' insert.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IHC-Fr
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

NMDA R, NR1 Subunit Antibody - Splice Variant Summary

• Description: The antigen is a peptide from the NR1 subunit, C2 splice variant insert of rat NMDA receptor.
• Immunogen: Synthetic peptide corresponding to amino acid residues specific to the NR1 subunit, C2 splice variant insert conjugated to KLH. Accession # P35439
• Specificity: Specific for the ~120k NR1 subunit of the NMDA Receptor containing the C2 splice variant insert. Does not recognize the NR1 subunits of the NMDA receptor that do not contain the C2 insert.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GRIN1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:1000-1:2000
  • Western Blot 1:1000
• Application Notes: By Western blot a band is seen at ~120 kDa representing the NMDAR1 C2 splice variant.
• Theoretical MW: 120 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Antigen Affinity-purified
• Reconstitution Instructions: Reconstitute with 50 ul PBS to desired concetration.

Alternate Names for NMDA R, NR1 Subunit Antibody - Splice Variant

• GluN1
• GRIN1
• MRD8
• NDHMSD
• NMDA R, NR1 Subunit
• NMDA1
• NMDAR1
• NMD-R1
• NR1

Background

The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA Receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). There are a number of different splice variants of the NR1 subunit (Foldes et al., 1994; Zukin and Bennett, 1995). Differential splicing of three exons in the NR1 subunit generates up to eight NR1 subunit splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2 prime). Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NMDA R, NR1 Subunit Antibody (NB300-116)(1)

Publication using NB300-116

• Nagy, J et al. Differential alterations in the expression of NMDA recaptor subunits following chromic ethanol treatment in primary cultures of rat cortical hippocampal neurons. Neurochemistry International, 42(2003):35-43. 2002-01-01 [PMID: 12441166]

Bioinformatics

• Gene Symbol: GRIN1
• Entrez:
  • 24408(Rat)
• Uniprot:
  • P35439(Rat)