NMDA R, NR1 Subunit Antibody - Splice Variant - Azide and BSA Free

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Western Blot: NMDAR2C Splice Variant Antibody [NB300-116] - Western blot of 10 ug of HEK 293 cells expression. (Lane A) NR1-subunit that does not contain the NR1 Insert (Mock). (Lane B) NR1-subunit containing only the ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

NMDA R, NR1 Subunit Antibody - Splice Variant - Azide and BSA Free Summary

Description
The antigen is a peptide from the NR1 subunit, C2 splice variant insert of rat NMDA receptor. Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).
Immunogen
Synthetic peptide corresponding to amino acid residues specific to the NR1 subunit, C2 splice variant insert conjugated to KLH. Accession # P35439
Specificity
Specific for endogenous levels of the ~120 kDa NR1 subunit of the NMDA Receptor containing the C2 splice variant insert. Does not recognize the NR1 subunits of the NMDA receptor that do not contain the C2 insert.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GRIN1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:1000-1:2000
  • Western Blot 1:1000
Application Notes
By Western blot a band is seen at ~120 kDa representing the NMDAR1 C2 splice variant.
Theoretical MW
120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NB300-116.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Preservative
No Preservative
Concentration
LYOPH
Purity
Antigen Affinity-purified
Reconstitution Instructions
Reconstitute with 50 ul PBS to desired concetration.

Alternate Names for NMDA R, NR1 Subunit Antibody - Splice Variant - Azide and BSA Free

  • GluN1
  • GRIN1
  • MRD8
  • NDHMSD
  • NDHMSR;NMD-R1
  • NMDA R, NR1 Subunit
  • NMDA1
  • NMDAR1
  • NMD-R1
  • NR1

Background

The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA Receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). There are a number of different splice variants of the NR1 subunit (Foldes et al., 1994; Zukin and Bennett, 1995). Differential splicing of three exons in the NR1 subunit generates up to eight NR1 subunit splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2 prime). Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NMDA R, NR1 Subunit Antibody (NB300-116)(1)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GRIN1
Entrez
Uniprot