NMDA R, NR1 Subunit Antibody (1031915) [Unconjugated]

GRIN1/NMDAR1 was detected in immersion fixed NS0 mouse myeloma cell line transfected with mouse GRIN1/NMDAR1 (positive staining) and NS0 mouse myeloma cell line (wild type, negative control) using Rat Anti-Mouse GRIN1/NMDAR1 Monoclonal Antibody (Catalog # MAB10655) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

NS0 cells transfected with mouse GRIN1/NMDAR1 (filled histogram) or irrelevant protein (open histogram) were stained with Rat Anti-Mouse GRIN1/NMDAR1 Monoclonal Antibody (Catalog # MAB10655) or Rat IgG2A Isotype Control Antibody (MAB006, data not shown) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012).

• Reactivity: Mu
• Applications: Flow, ICC/IF
• Clone: 1031915
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

NMDA R, NR1 Subunit Antibody (1031915) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse GRIN1/NMDAR1
  Met1-Gln559
• Specificity: Detects mouse GRIN1/NMDAR1 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NMDA R, NR1 Subunit Antibody (1031915) [Unconjugated]

• GluN1
• GRIN1
• MRD8
• NDHMSD
• NDHMSR;NMD-R1
• NMDA R, NR1 Subunit
• NMDA1
• NMDAR1
• NMD-R1
• NR1

Background

Grin-1 is a critical component of NMDA receptors.  Expressed in the brain, these components play a key role in plasticity of synapses, which is believed to underlie memory and learning.  Missense variants of the receptor components cause similar syndromes with varying severity of intellectual impairment, autism, epilepsy, and motor dysfunction.  In Mice with reduced NMDA receptor activity, schizophrenia-like behaviors are revealed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.