NM23-H1 Antibody (2H1)

Western Blot: NM23-H1 Antibody (2H1) [H00004830-M01] - Analysis of NME1 expression in transfected 293T cell line by NME1 monoclonal antibody (M01), clone 2H1.Lane 1: NME1 transfected lysate(19.7 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: NM23-H1 Antibody (2H1) [H00004830-M01] - Analysis of monoclonal antibody to NME1 on HeLa cell. Antibody concentration 20 ug/ml.

Western Blot: NM23-H1 Antibody (2H1) [H00004830-M01] - Analysis of NME1 over-expressed 293 cell line, cotransfected with NME1 Validated Chimera RNAi ( Cat # H00004830-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NME1 monoclonal antibody (M01), clone 2H1 (Cat # H00004830-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: NM23-H1 Antibody (2H1) [H00004830-M01] - NME1 monoclonal antibody (M01), clone 2H1 Analysis of NME1 expression in Hela S3 NE.

ELISA: NM23-H1 Antibody (2H1) [H00004830-M01] - Detection limit for recombinant GST tagged NME1 is approximately 1ng/ml as a capture antibody.

Product Discontinued

NM23-H1 Antibody (2H1) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: NME1 (AAH00293, 1 a.a. ~ 152 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MANCERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVGLKFMQASEDLLKEHYVDLKDRPFFAGLVKYMHSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVESAEKEIGLWFHPEELVDYTSCAQNWIYE
• Specificity: NME1 - non-metastatic cells 1, protein (NM23A) expressed in
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NME1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NM23-H1 Antibody (2H1)

• EC 2.7.4.6
• GAAD
• Granzyme A-activated DNase
• Metastasis inhibition factor nm23
• NB
• NBS
• NDK A
• NDKA
• NDP kinase A
• NDPKA
• NDPK-A
• NM23A
• NM23AWD
• NM23H1
• NM23-H1
• NME1
• non-metastatic cells 1, protein (NM23A) expressed in
• nucleoside diphosphate kinase A
• Tumor metastatic process-associated protein

Background

This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NME1
• Entrez:
  • 4830(Human)
• Uniprot:
  • AAH00293(Human)