NLRP2/NALP2 Antibody

Immunohistochemistry-Paraffin: NLRP2/NALP2 Antibody [NBP2-24667] - Analysis of NALP2 in formalin-fixed, paraffin-embedded human colon tumor tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.

Product Discontinued

NLRP2/NALP2 Antibody Summary

• Immunogen: A portion of amino acids 1030-1080 of human NALP2 was used as the immunogen.
• Predicted Species: Mouse (100%), Rat (100%), Chicken (100%), Canine (100%), Equine (100%), Zebrafish (100%), Bovine (100%), Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NLRP2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot
• Application Notes: Use in Western blot reported in scientific literature (PMID 21483755)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for NLRP2/NALP2 Antibody

• CLR19.9
• FLJ20510
• NACHT, leucine rich repeat and PYD containing 2
• NACHT, LRR and PYD domains-containing protein 2
• NALP2
• NALP2NACHT, LRR and PYD containing protein 2
• NBS1
• NBS1PYRIN-containing APAF1-like protein 2
• NLR family, pyrin domain containing 2
• NLRP2
• nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domaincontaining 2
• PAN1
• PAN1Nucleotide-binding site protein 1
• PYPAF2
• PYPAF2PYRIN domain and NACHT domain-containing protein 1

Background

NLRP2/NALP2, aka PAN1 and PYPAF2, belongs to a cytoplasmic proteins family containing a NACHT domain, leucine rich repeat (NLR) and N-terminal pyrin-containing domain (PYD). As an intracellular pathogen-recognition receptors (PRRs), NLRP2/NALP2 is a 1062 amino acid protein associated in cell responses to apoptotic and inflammatory stimuli. PRRs are key components of immune systems involving in an innate effector mechanisms and activation of adaptive immunity. By interacting with ASC in addition to CARD8 and caspase 1, NLRP2/NALP2 forms an inflammasome functioning as a modulator of NF-kB and procaspase-1 activation in macrophages. Similar to TLRs, activation of the inflammasome by NLRs occurs through the recognition of pathogen-associated molecular patterns (PAMPs) through their LRR domains. NLRP2/NALP2 expressions varies in several human tumor cell lines though the highest were found in MDA-MB-435 and MCF-7 breast cancer, UACC62 melanoma, and Caco2 colon cancer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NLRP2/NALP2 Antibody (NBP2-24667)(2)

Publications using NBP2-24667

• Geng F, Ma Y, Xing T et al. Effects of Hyperbaric Oxygen Therapy on Inflammasome Signaling after Traumatic Brain Injury. Neuroimmunomodulation. 2016-05-24 [PMID: 27216735]
• Menendez Daniel, Shatz Maria, Azzam Kathleen et al. The Toll-like receptor gene family is integrated into human DNA damage and p53 networks. PLoS Genet. 2011-03-01 [PMID: 21483755] (WB, Human)

Bioinformatics

• Gene Symbol: NLRP2
• Uniprot:
  • Q9NX02()