NLRP1/NALP1 Antibody (Nalpy1-4) - BSA Free

Western Blot: NLRP1/NALP1 Antibody (Nalpy1-4) [NBP1-97593] - Analysis of the time course of assembly of the inflammasome using MAb to NLRP1/NALP1 (Nalpy1-4) in THP-1 macrophages. Method: Assembly of the inflammasome was induced by shifting the temperature to 30C after hypotonic lysis. THP-1 cell extracts were immunoprecipitated using PAb to Asc (AL177) and run on SDS-PAGE. NLRP1/NALP1 was detected by MAb to NALP1 (Nalpy1-4) at 1:1000 dilution. Anti-mouse IgG coupled horse radish peroxidase was used at 1:5000 dilution for ECL detection. For more information about the 'time course assembly of inflammasome' method see F. Martinon; Mol. Cell. 10, 417 (2002).

Immunocytochemistry/Immunofluorescence: NALP1 Antibody (Nalpy1-4) [NBP1-97593] - Detection of NLRP1/NALP1 in 293T cells transfected with a human NLRP1/NALP1 expression plasmid. Left: Phase contrast. Right: Staining with MAb to NLRP1/NALP1 (Nalpy1-4).Method: 293T cells transfected with full length human NLRP1/NALP1 were fixed with 4% paraformaldehyde and stained with 1ug/ml MAb to NLRP1/NALP1 (Nalpy1-4) and then Alexa 488 (1:300 dilution).

Product Discontinued

NLRP1/NALP1 Antibody (Nalpy1-4) - BSA Free Summary

• Immunogen: Recombinant human NLRP1/NALP1 (NACHT-, LRR- and PYD-containing protein 1) (pyrin domain).
• Specificity: Recognizes the pyrin domain (PYD) of human NLRP1/NALP1..
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NLRP1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:1000

Reactivity Notes

Does not cross-react with mouse NLRP1/NALP1.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for NLRP1/NALP1 Antibody (Nalpy1-4) - BSA Free

• CARD7
• CARD7SLEV1
• Caspase recruitment domain-containing protein 7
• CLR17.1
• Death effector filament-forming ced-4-like apoptosis protein
• DEFCAP
• DEFCAPVAMAS1
• DKFZp586O1822
• KIAA0926DEFCAP-L/S
• NACHT, leucine rich repeat and PYD (pyrin domain) containing 1
• NACHT, leucine rich repeat and PYD containing 1
• NACHT, LRR and PYD containing protein 1
• NACHT, LRR and PYD domains-containing protein 1
• NACPP1044
• NALP1
• NALP1caspase recruitment domain protein 7
• NLR family, pyrin domain containing 1
• NLRP1
• Nucleotide-binding domain and caspase recruitment domain
• nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domaincontaining 1
• PP1044
• SLEV1
• VAMAS1

Background

NALP1 (also known as NAC/CARD7) is a member of the CATERPILLER (CLR) diverse multigene superfamily of immune regulatory proteins (Ting and Davis, 2005). CLR proteins have a variable N-terminal domain, followed by a nucleotide-binding domain (NBD) and leucine-rich repeats (LRR). The N-terminal domain consists of transactivation, CARD, Pyrin or BIR domains, or in some cases is undefined. The CLR family, also known as the NOD family, has its ancient roots in the plant kingdom and is related to the disease resistance (R) gene family that mediates plant immune responses. CLR proteins participate in both innate and adaptive immunity in mammals, some act as sensors that detect pathogen products, and several CLR genes have been linked to susceptibility to immunological diseases. It is thought that the CLR family may have a fundamental importance for the function of the mammalian innate/ancient immune system on par with the Toll-like receptor (TLR) family. NALP1, a member of NALP CLR subfamily, has an N-terminal pyrin domain (PYD), followed by an NBD, LRR, and a C-terminal CARD domain (reviewed in Tschopp. NALP1 is a component of both the inflammasome and apoptosome, suggesting that NALP1 it has important roles in both inflammation and apoptosis. This antibody recognizes NALP1; human NALP1 is a 1473 amino acid protein and migrates at approx. 166 kDa on SDS-PAGE. However, multiple splice variants encoding distinct NALP1 isoforms with varying amino acid lengths have been described and thus the molecular weight detected may vary depending on the isoform(s) expressed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NLRP1/NALP1 Antibody (NBP1-97593)(5)

Publications using NBP1-97593

• Bi F, Bai Y, Zhang Y, Liu W Ligustroflavone exerts neuroprotective activity through suppression of NLRP1 inflammasome in ischaemic stroke mice Experimental and therapeutic medicine 2023-01-01 [PMID: 36561613] (WB, Mouse)
• M�h�nen K, Hau A, Bondet V et al. Activation of NLRP3 Inflammasome in the Skin of Patients with Systemic and Cutaneous Lupus Erythematosus Acta Dermato-Venereologica 2022-05-10 [PMID: 35356994]
• Verma D, Fekri SZ, Sigurdardottir G et al. Enhanced inflammasome activity in patients with psoriasis promotes systemic inflammation J. Invest. Dermatol. 2020-09-04 [PMID: 32896537]
• Bhardwaj S, Rani S, Kumaran MS et al. Expression of Th17- and Treg-specific transcription factors in vitiligo patients Int. J. Dermatol. 2020-01-07 [PMID: 31909498]
• JA Kummer et al. Inflammasome components NALP 1 and 3 show distinct but separate expression profiles in human tissues suggesting a site-specific role in the inflammatory response. J. Histochem. Cytochem. 55, 443. 2007-01-01 [PMID: 17164409]

Bioinformatics

• Gene Symbol: NLRP1