NKX3.1 Antibody (4H4)

Western Blot: NKX3.1 Antibody (4H4) [NBP1-51609] - Analysis using NKX3A mouse mAb against LNCaP (1) cell lysate.

Immunohistochemistry-Paraffin: NKX3.1 Antibody (4H4) [NBP1-51609] - Analysis of human prostata tissues (A, B) using anti-NKX3A antibody with DAB staining.

Flow Cytometry: NKX3.1 Antibody (4H4) [NBP1-51609] - Analysis of PC-3 cells using anti-NKX3A mAb (green) and negative control (purple).

Immunohistochemistry-Paraffin: NKX3.1 Antibody (4H4) [NBP1-51609] - Analysis of human Liver tissues using NKX3A mAb

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC
• Clone: 4H4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

NKX3.1 Antibody (4H4) Summary

• Description: Novus Biologicals Mouse NKX3.1 Antibody (4H4) (NBP1-51609) is a monoclonal antibody validated for use in IHC, WB, ELISA and Flow. Anti-NKX3.1 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human NKX3.1 expressed in E. Coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NKX3-1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 26.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for NKX3.1 Antibody (4H4)

• BAPX2
• BAPX2homeobox protein Nkx-3.1
• Homeobox protein NK-3 homolog A
• NK homeobox (Drosophila), family 3, A
• NK3 homeobox 1
• NK3 transcription factor homolog A
• NK3 transcription factor related, locus 1 (Drosophila)
• NK3 transcription factor related, locus 1
• NKX3.1
• NKX3.1NKX3
• NKX3-1
• NKX3A
• NKX3ANK homeobox, family 3, A

Background

Nkx3.1 is a transcription factor that may play an important role in regulating proliferation of glandular epithelium and in the formation of ducts in the prostate. It has been thought to be one of the target genes of the 8p21 loss of heterozygosity, common in prostate cancer. But neither disruption of the coding region of the gene, nor mutations have been found in prostate cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NKX3.1 Antibody (NBP1-51609)(3)

Publications using NBP1-51609

• Mol Biol Rep. 37(3):1505-12. 2010-03-01 [PMID: 19462257]
• Exp Biol Med (Maywood). 233(3):297-309. 2008-03-01 [PMID: 18296735]
• Jiang A, Yu C, Zhang P et al. p53 overexpression represses androgen-mediated induction of NKX3.1 in a prostate cancer cell line. Exp Mol Med. 38(6):625-33. 2006-12-31 [PMID: 17202838]

Bioinformatics

• Gene Symbol: NKX3-1
• Entrez:
  • 4824(Human)
• Uniprot:
  • Q99801(Human)