NKX2.5 Antibody

Images

 
Western Blot: NKX2.5 Antibody [NBP1-51953] - Staining of Mouse Heart lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: NKX2.5 Antibody [NBP1-51953] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, ICC/IF, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

NKX2.5 Antibody Summary

Immunogen
Peptide with sequence C-PRAYSDPDPAKDPR corresponding to internal region according to NP_001159647.1, NP_001159648.1.
Epitope
C-PRAYSDPDPAKDPR
Marker
Cardiac Progenitor Marker
Specificity
This antibody is expected to recognize all three reported isoforms (NP_004378.1; NP_001159647.1; NP_001159648.1).
Predicted Species
Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NKX2-5
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.5 ug/mL
  • Immunocytochemistry/Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:32000
Application Notes
Approx 40-45kDa band observed in Mouse Heart lysates (calculated MW of 34.2kDa according to NP_032726.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Shiojima et al, Circ Res. 1996 Nov;79(5):920-9.; PMID: 8888684).
Publications
Read Publication using NBP1-51953.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NKX2.5 Antibody

  • cardiac-specific homeo box
  • cardiac-specific homeobox 1
  • Cardiac-specific homeobox
  • CHNG5
  • CSX1
  • CSXFLJ97195
  • FLJ52202
  • FLJ99536
  • Homeobox protein CSX
  • Homeobox protein NK-2 homolog E
  • homeobox protein Nkx-2.5
  • NK2 transcription factor related, locus 5 (Drosophila)
  • NKX2.5
  • NKX2.5FLJ97166
  • NKX2-5
  • NKX2E
  • NKX2EFLJ97197
  • NKX4-1
  • tinman paralog

Background

Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development (Shiojima et al., 1995 [PubMed 7665173]). It has been demonstrated that a Drosophila homeobox-containing gene called 'tinman' is expressed in the developing dorsal vessel and in the equivalent of the vertebrate heart. Mutations in tinman result in loss of heart formation in the embryo, suggesting that tinman is essential for Drosophila heart formation. Furthermore, abundant expression of Csx, the presumptive mouse homolog of tinman, is observed only in the heart from the time of cardiac differentiation. CSX, the human homolog of murine Csx, has a homeodomain sequence identical to that of Csx and is expressed only in the heart, again suggesting that CSX plays an important role in human heart formation.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NKX2.5 Antibody (NBP1-51953)(1)


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Bioinformatics

Gene Symbol NKX2-5
Entrez
Uniprot