NKp44/NCR2 Antibody (253415) [Allophycocyanin]

Images

 
Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with Recombinant Human IL‑2 (Catalog # 202-IL) were stained with Mouse Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
253415
Clonality
Monoclonal
Host
Mouse
Conjugate
Allophycocyanin

NKp44/NCR2 Antibody (253415) [Allophycocyanin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human NKp44/NCR2
Gln22-Pro190
Accession # CAB52289
Specificity
Detects human NKp44/NCR2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) NKp30, rhNKp46, rhNKp80, or recombinant mouse NKp46 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
NCR2
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells
Reviewed Applications
Read 2 Reviews rated 5
using
FAB22491A in the following application:

Publications
Read Publication using
FAB22491A in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide
Purity
Protein A or G purified from hybridoma culture supernatant

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKp44/NCR2 Antibody (253415) [Allophycocyanin]

  • CD336 antigen
  • CD336
  • LY95
  • LY95lymphocyte antigen 95 homolog (activating NK-receptor; NK-p44)
  • lymphocyte antigen 95 (activating NK-receptor; NK-p44)
  • Lymphocyte antigen 95 homolog
  • natural cytotoxicity triggering receptor 2
  • Natural killer cell p44-related protein
  • NCR2
  • NK cell activating receptor (NKp44)
  • NK cell-activating receptor
  • NKp44
  • NK-p44dJ149M18.1

Background

NKp44, along with NKp30 and NKp46, constitute a group of receptors termed "Natural Cytotoxicity Receptors" (NCR) (1). These receptors are expressed almost exclusively by NK cells and play a major role in triggering NK-mediated killing of most tumor cell lines. No rodent ortholog to NKp44 has been identified. Human NKp44, also known as NCR2, is a 44 kDa type I transmembrane glycoprotein that is characterized by the presence of one extracellular V-like immunoglobulin domain (2). It is synthesized as a 276 amino acid (aa) precursor that contains a 21 aa signal sequence, a 171 aa extracellular region, a 21 aa transmembrane segment and a 63 aa cytoplasmic tail. Alternate splicing in both the cytoplasmic tail and extracellular region generates multiple isoforms of unknown significance. The Ig-like region is unaffected. A physical association with the ITAM-bearing accessory protein, DAP12, occurs via a charged residue in the NKp44 transmembrane domain. Ligation of NKp44 with a specific antibody results in phosphorylation of DAP12 (3) and activation of target cell lysis in a redirected killing assay (4). NKp44 is absent from resting NK cells but is upregulated upon activation with IL-2. Activation-induced expression occurs in the CD56dim CD16+ NK subset that accounts for more than 85% of NK cells found in peripheral blood and spleen, as well as the CD56bright CD16- NK subset that constitutes the majority of NK cells in lymph node and tonsil (5). Studies with neutralizing antibodies reveal that NKp44 is partially responsible for triggering lytic activity against several tumor cell types (2, 6). Blocking any of the individual NCRs results in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis is observed if all three receptors are blocked simultaneously (6). NKp44 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (7).

  1. Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
  2. Cantoni, C. et al. (1999) J. Exp. Med. 189:787.
  3. Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235.
  4. Vitale, M. et al. (1998) J. Exp. Med. 187:2065.
  5. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
  6. Pende, D. et al. (1999) J. Exp. Med. 190:1505.
  7. Arnon, T. et al. (2001) Eur. J. Immunol. 31:2680.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu
Applications: Flow

Publications for NKp44/NCR2 Antibody (FAB22491A)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow.


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Reviews for NKp44/NCR2 Antibody (FAB22491A) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using FAB22491A:
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Flow
(2)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
Flow Human 01/22/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID: 22084431
SpeciesHuman

Blocking

Blocking DetailsSee PMID: 22084431

Details

Detection NotesSee PMID: 22084431

Comments

CommentsIndependent review from antibodyresource.com
 
reviewed by:
Anonymous
Flow Human 01/22/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID: 22084431
SpeciesHuman

Blocking

Blocking DetailsSee PMID: 22084431

Details

Detection NotesSee PMID: 22084431

Comments

CommentsIndependent review from antibodyresource.com

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

 
Anonymous
01/22/2015
Application: Flow
Species: Human

 
Anonymous
01/22/2015
Application: Flow
Species: Human

Bioinformatics

Gene Symbol NCR2
Uniprot