NKIRAS2 Overexpression Lysate

Images

 
Western Blot: NKIRAS2 Overexpression Lysate (Adult Normal) [NBL1-13656] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NKIRAS2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

NKIRAS2 Overexpression Lysate Summary

Description

NKIRAS2 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC209464

Accession#: NM_017595

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
NKIRAS2

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct. Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
21.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for NKIRAS2 Overexpression Lysate

  • DKFZP434N1526
  • I-kappa-B-interacting Ras-like protein 2
  • Kappa B-Ras protein 2
  • kappaB-Ras2
  • KBRAS2DKFZp434N1526
  • MGC74742
  • NF-kappa-B inhibitor-interacting Ras-like protein 2
  • NFKB inhibitor interacting Ras-like 2
  • NFKB inhibitor interacting Ras-like protein 2

Background

Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and nuclear localization of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56583
Species: Hu, Rb, Rt
Applications: Flow, IHC, IHC-P, KO, Simple Western, WB
NB100-56144
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
NB100-56555
Species: Bv, Ca, Eq, Hu, Mu, Po, Pm, Rt, Xp
Applications: WB
NB100-55735
Species: Bv, Ca, Ch, Hu, Mu, Pm
Applications: ICC/IF, IP, WB
NBP2-14873
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP2-22106
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
NBP2-29373
Species: Hu, Mu, Rt
Applications: Flow-CS, Flow, ICC/IF
6507-IL/CF
Species: Hu
Applications: BA
AF2377
Species: Mu
Applications: IHC, Simple Western, WB
AF3667
Species: Hu, Mu
Applications: Dual ISH-IHC, ICC, IHC, Simple Western, WB
AF773
Species: Hu
Applications: CyTOF-ready, Flow, Neut, WB
NBP2-50071
Species: Hu
Applications: CyTOF-ready, Flow, ICC/IF, IF, IHC, IHC-P
NBP2-67429
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP2-61706
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP3-35880
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, WB
AF942
Species: Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow, WB
NBP1-87185
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
AF1927
Species: Hu
Applications: IP, WB
NBP2-03143
Species: Ca, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB

Publications for NKIRAS2 Lysate (NBL1-13656) (0)

There are no publications for NKIRAS2 Lysate (NBL1-13656).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for NKIRAS2 Lysate (NBL1-13656) (0)

There are no reviews for NKIRAS2 Lysate (NBL1-13656). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for NKIRAS2 Lysate (NBL1-13656) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional NKIRAS2 Products

Research Areas for NKIRAS2 Lysate (NBL1-13656)

Find related products by research area.

Blogs on NKIRAS2

There are no specific blogs for NKIRAS2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our NKIRAS2 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol NKIRAS2