NKIRAS1 Antibody (184C278)

Images

 
Western Blot: NKIRAS1 Antibody (184C278) [NB100-56555] - Analysis of human 293 cell lysate probed using 0.5 ug/ml. Image using the Azide Free form of this antibody.
Western Blot: NKIRAS1 Antibody (184C278) [NB100-56555] - Analysis of NKIRAS1 using this antibody. Human 293 cell lysate probed with kB-Ras1 antibody at 0.5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Eq, Pm, XpSpecies Glossary
Applications WB
Clone
184C278
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

NKIRAS1 Antibody (184C278) Summary

Immunogen
This monoclonal antibody was raised against a synthetic peptide containing amino acid residues 96-113 (RVELLKKEIDKFKDKKEV) of human kB-Ras1.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NKIRAS1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1 ug/ml
Control
293 Whole Cell Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.2% gelatin
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for NKIRAS1 Antibody (184C278)

  • I-kappa-B-interacting Ras-like protein 1
  • kappa B-ras 1
  • Kappa B-Ras protein 1
  • KappaB-Ras1
  • KBRAS1kappaB-Ras1
  • NF-kappa-B inhibitor-interacting Ras-like protein 1
  • NFKB inhibitor interacting Ras-like 1
  • NFKB inhibitor interacting Ras-like protein 1

Background

NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified. One of the first genes induced following NF-kB activation is IkBa. Recently, two proteins, kB-Ras1 and kB-Ras2 have been identified. These two proteins interact with PEST domains of IkBa and IkBb and decrease their rate of degradation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for NKIRAS1 Antibody (NB100-56555) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NB100-56555AF405
Alexa Fluor 488 NB100-56555AF488
Alexa Fluor 532 NB100-56555AF532
Alexa Fluor 647 NB100-56555AF647
Alexa Fluor 700 NB100-56555AF700
Biotin NB100-56555B
DyLight 350 NB100-56555UV
DyLight 405 NB100-56555V
DyLight 488 NB100-56555G
DyLight 550 NB100-56555R
DyLight 594 NB100-56555DL594
DyLight 650 NB100-56555C
DyLight 680 NB100-56555FR
DyLight 755 NB100-56555IR
FITC NB100-56555F
HRP NB100-56555H

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Bioinformatics

Gene Symbol NKIRAS1
Entrez
Uniprot
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