NKIRAS2 Antibody (2G9)

Western Blot: NKIRAS2 Antibody (2G9) [H00028511-M02] - NKIRAS2 monoclonal antibody (M02), clone 2G9 Analysis of NKIRAS2 expression in HeLa.

Immunohistochemistry-Paraffin: NKIRAS2 Antibody (2G9) [H00028511-M02] - Analysis of monoclonal antibody to NKIRAS2 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.

Sandwich ELISA: NKIRAS2 Antibody (2G9) [H00028511-M02] - Detection limit for recombinant GST tagged NKIRAS2 is approximately 1ng/ml as a capture antibody.

Product Discontinued

NKIRAS2 Antibody (2G9) Summary

• Immunogen: NKIRAS2 (AAH07450, 1 a.a. ~ 191 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGKSCKVVVCGQASVGKTSILEQLLYGNHVVGSEMIETQEDIYVGSIETDRGVREQVRFYDTRGLRDGAELPRHCFSCTDGYVLVYSTDSRESFQRVELLKKEIDKSKDKKEVTIVVLGNKCDLQEQRRVDPDVAQHWAKSEKVKLWEVSVADRRSLLEPFVYLASKMTQPQSKSAFPLSRKNKGSGSLDG
• Specificity: NKIRAS2 - NFKB inhibitor interacting Ras-like 2
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NKIRAS2
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Ascites

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NKIRAS2 Antibody (2G9)

• DKFZP434N1526
• I-kappa-B-interacting Ras-like protein 2
• Kappa B-Ras protein 2
• kappaB-Ras2
• KBRAS2DKFZp434N1526
• MGC74742
• NF-kappa-B inhibitor-interacting Ras-like protein 2
• NFKB inhibitor interacting Ras-like 2
• NFKB inhibitor interacting Ras-like protein 2

Background

Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and nuclear localization of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NKIRAS2 Antibody (H00028511-M02)(1)

Publication using H00028511-M02

• Murphy AJ, Guyre PM, Pioli PA. Estradiol suppresses NF-kappa B activation through coordinated regulation of let-7a and miR-125b in primary human macrophages. J Immunol 184(9):5029-37. 2010-05-01 [PMID: 20351193]

Bioinformatics

• Gene Symbol: NKIRAS2
• Entrez:
  • 28511(Human)
• OMIM:
  • 604497(Human)
• Uniprot:
  • AAH07450(Human)