NKG2D/CD314 Antibody (2855A) [Unconjugated]

Day 10 activated and expanded NK cells with CD56 costain treated with 27 ng/ml Recombinant Human IL-2 Protein (202-IL), 10 ng/ml Recombinant Human IL-12 Protein (219-IL), 10 ng/ml Recombinant Human IL-18/IL-1F4 Protein (9124-IL) and 10ng/ml Recombinant Human IL-21 Protein (8879-IL) for 10 days were stained with either (A) Rabbit Anti-Human NKG2D/CD314 Monoclonal Antibody (Catalog # MAB1391) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 2855A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

NKG2D/CD314 Antibody (2855A) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Mouse myeloma cell line, NS0-derived human NKG2D/CD314
  Phe78-Val216
  Accession # P26718
• Specificity: Detects human NKG2D/CD314 in direct ELISA.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKG2D/CD314 Antibody (2855A) [Unconjugated]

• CD314 antigen
• CD314
• D12S2489E
• FLJ17759
• FLJ75772
• Killer cell lectin-like receptor subfamily K member 1
• killer cell lectin-like receptor subfamily K, member 1
• KLR
• KLRK1
• NK cell receptor D
• NKG2-D type II integral membrane protein
• NKG2D
• NKG2-D
• NKG2-D-activating NK receptor
• NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence

Background

NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8⁺ alpha beta T cells, gamma δ T cells, NK cells and NKT cells. In mouse systems NKG2D also occurs on macrophages. Human ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3. Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection. NKG2D exists as a disulfide-linked homodimer that delivers an activating signal upon ligand binding. Signaling requires association with an adapter protein. Alternative splicing of the NKG2D mRNA results in isoforms with different cytoplasmic domains that can associate either with DAP12 to deliver a true activating signal or with DAP10 resulting in a costimulatory signal. NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection.

1. Li, P. et al. (2001) Nature Immunol. 2:443.
2. Steinle, A. et al. (2001) Immunogenetics 53:279.
3. Cosman, D. et al. (2001) Immunity 14:123.
4. Cerwenka, A. and L. Lanier (2001) Immunol. Rev. 181:158.
5. Wu, J. et al. (1999) Science 285:730.
6. Diefenbach, A. et al. (2002) Nature Immunol. 3:1142.
7. Gilfillan, S. et al. (2002) Nature Immunol. 3:1150.
8. Groh, V. et al. (2001) Nature Immunol. 2:255.
9. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
10. Diefenbach, A. et al. (2001) Nature 413:165.
11. NKG2D and its Ligands (2002) www.rndsystems.com

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P26718()