NKG2D/CD314 Antibody (149810) [Alexa Fluor® 488]

Peripheral blood mononuclear cells (PBMCs) gated on CD3-cells were stained with Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P) and either (A) Mouse Anti-Human NKG2D/CD314 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB139G) or (B) Mouse IgG1Alexa Fluor 488 Isotype Control (Catalog # IC002G). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 149810
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

NKG2D/CD314 Antibody (149810) [Alexa Fluor® 488] Summary

• Additional Information: Clone 149810 was used by HLDA to establish CD designation
• Immunogen: BaF3 mouse pro-B cell line transfected with human NKG2D/CD314
• Specificity: Detects human NKG2D/CD314 in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KLRK1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 5 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• 12 months, 2 to 8 °C under sterile conditions after opening.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKG2D/CD314 Antibody (149810) [Alexa Fluor® 488]

• CD314 antigen
• CD314
• D12S2489E
• FLJ17759
• FLJ75772
• Killer cell lectin-like receptor subfamily K member 1
• killer cell lectin-like receptor subfamily K, member 1
• KLR
• KLRK1
• NK cell receptor D
• NKG2-D type II integral membrane protein
• NKG2D
• NKG2-D
• NKG2-D-activating NK receptor
• NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence

Background

NKG2D, also known as CD314, is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C‑type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8⁺ alpha beta T cells, gamma δ T cells, NK cells, and NKT cells. In mouse systems NKG2D also occurs on macrophages. Human ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3. Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection. NKG2D exists as a disulfide-linked homodimer that delivers an activating signal upon ligand binding. Signaling requires association with an adapter protein. Alternative splicing of the NKG2D mRNA results in isoforms with different cytoplasmic domains that can associate either with DAP12 to deliver a true activating signal or with DAP10 resulting in a costimulatory signal. NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection.

1. Li, P. et al. (2001) Nature Immunol. 2:443.
2. Steinle, A. et al. (2001) Immunogenetics 53:279.
3. Cosman, D. et al. (2001) Immunity 14:123.
4. Cerwenka, A. and L. Lanier (2001) Immunol. Rev. 181:158.
5. Wu, J. et al. (1999) Science 285:730.
6. Diefenbach, A. et al. (2002) Nature Immunol. 3:1142.
7. Gilfillan, S. et al. (2002) Nature Immunol. 3:1150.
8. Groh, V. et al. (2001) Nature Immunol. 2:255.
9. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
10. Diefenbach, A. et al. (2001) Nature 413:165.
11. NKG2D and its Ligands (2002) www.RnDSystems.com.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NKG2D/CD314 Antibody (FAB139G)(4)

Publications using FAB139G

• Xu Geng, Ming Li, Bin Cui, Chao Lu, Xiaowen Liu, Peng Zhang et al. CD4+CD25+Foxp3+ regulatory T cells suppress NKG2D-mediated NK cell cytotoxicity in peripheral blood Medicine (Baltimore) 5/31/2019 [PMID: 31145286]
• K Goto, J Arai, A Stephanou, N Kato Novel therapeutic features of disulfiram against hepatocellular carcinoma cells with inhibitory effects on a disintegrin and metalloproteinase 10 Oncotarget, 2018-04-10;9(27):18821-18831. 2018-04-10 [PMID: 29721164] (Flow Cytometry, Human)
• Wei-Chen Huang T Cells Infiltrating Diseased Liver Express Ligands for the NKG2D Stress Surveillance System J. Immunol, 2016-12-28;0(0):. 2016-12-28 [PMID: 28031333] (Flow Cytometry, Human)
• Novel chemoimmunotherapeutic strategy for hepatocellular carcinoma based on a genome-wide association study Sci Rep, 2016-12-02;6(0):38407. 2016-12-02 [PMID: 27910927] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: KLRK1