Ninjurin-1 Antibody (758926) [Alexa Fluor® 488]

HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Ninjurin-1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB51051G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 758926
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Ninjurin-1 Antibody (758926) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human Ninjurin-1
  Asp2-Val81
  Accession # Q92982
• Specificity: Detects human Ninjurin-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Ninjurin-2 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NINJ1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ninjurin-1 Antibody (758926) [Alexa Fluor® 488]

• Nerve injury-induced protein 1
• nerve injury-induced protein-1
• NIN1
• NINJ1
• ninjurin 1
• NINJURIN
• Ninjurin1
• Ninjurin-1

Background

Ninjurin-1 (Nerve Injury-induced Protein 1) is a 20-22 kDa phosphoprotein member of the Ninjurin family of transmembrane (TM) proteins. It is either constitutively or inducibly expressed by Schwann cells, PNS neuron satellite cells, neutrophils, endothelial cells, macrophages, podocytes and hepatocytes, and participates in intercellular homophilic binding during nerve regeneration. Human Ninjurin-1 is 152 amino acids in length. It has an unusual membrane orientation. There is an 80 amino acid (aa) N-terminal extracellular domain (ECD) (aa 1-80), followed by a TM segment, a cytoplasmic region, a second TM segment and a C-terminal ECD (aa 142-152). Homophilic binding is divalent-cation dependent and occurs between Pro26 and Asn37. Over aa 1-80, human Ninjurin-1 shares 84% aa sequence identity with mouse Ninjurin-1. With respect to human Ninjurin-2, which has only 65 aa in it N-terminal ECD, human Ninjurin-1 shares 51% aa sequence identity with human Ninjurin-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NINJ1
• Uniprot:
  • Q92982()