Ninein Antibody

Western Blot: Ninein Antibody [NB100-74631] - DGAT1 inhibition decreases ncMTOC-related proteins in prostate cancer cells. gamma-tubulin (48 kD), ninein (243 kD), GM130 (140 kD), and CETN1 (20 kD) levels were analyzed by western blot in PC-3 control (CTR) and treated with 1 uM DGAT1 inhibitor or 10 nM PEDF. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-39537-z), licensed under a CC-BY license.

Western Blot: Ninein Antibody [NB100-74631] - Detection of Human and Mouse NINEIN on HeLa whole cell lysate using NB100-74631. NINEIN was also immunoprecipitated by another rabbit anti-NINEIN antibody.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Ninein Antibody Summary

• Description: Novus Biologicals Rabbit Ninein Antibody (NB100-74631) is a polyclonal antibody validated for use in WB and IP. Anti-Ninein Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1850 and 1900 of human Ninein using the numbering given in entry NP_891991.1 (GeneID 51199).
• Marker: Centrosome Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NIN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Theoretical MW: 243 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Ninein Antibody

• Glycogen synthase kinase 3 beta-interacting protein
• GSK3B-interacting protein
• hNinein
• KIAA1565
• ninein (GSK3B interacting protein)
• ninein centrosomal protein
• ninein

Background

This gene encodes one of the proteins important for centrosomal function. This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells. Localization of this protein to the centrosome requires three leucine zippers in the central coiled coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ninein Antibody (NB100-74631)(1)

Publication using NB100-74631

• Nardi F, Franco OE, Fitchev P et al. DGAT1 Inhibitor Suppresses Prostate Tumor Growth and Migration by Regulating Intracellular Lipids and Non-Centrosomal MTOC Protein GM130 Sci Rep 2019-02-28 [PMID: 30816200] (WB, Human)

Review for Ninein Antibody (NB100-74631) (1)

Average Rating: 1

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer ICC Mouse 08/16/2021

Summary

• Application: Immunocytochemistry
• Sample Tested: Adult small intestine
• Species: Mouse
• Lot: A1

Comments

• Comments: The sample is a smear (cytology-like preps) of mouse small intestinal epithelial cells fixed with 2% paraformaldehyde/lysine/periodate (PLP; McLean and Nakane. J Histochem Cytochem, 1974). Results were similar after fixation with -20°C methanol. After permeabilizing with 0.1% saponin (PLP-fixed only) and blocking with 5% donkey serum (in PBS), slides were incubated with antibodies at the dilutions indicated below and a rat monoclonal anti-ZO-1 (tight junction protein) antibody (clone R40.76) for 2 hours at room temperature. After washing, species-specific secondary antibodies (cross-adsorbed) produced in goat were applied. ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

Bioinformatics

• Gene Symbol: NIN
• Entrez:
  • 51199(Human)
  • 18080(Mouse)
• Uniprot:
  • Q8N4C6(Human)