Nidogen-1/Entactin Antibody

Western Blot: Nidogen-1/Entactin Antibody [NBP2-16341] - Sample (30 ug of whole cell lysate) A: NT2D1 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: Nidogen-1/Entactin Antibody [NBP2-16341] - Human liver carcinoma, using Nidogen antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Nidogen-1/Entactin Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Nidogen-1/Entactin. The exact sequence is proprietary.
• Localization: Secreted; extracellular space; extracellular matrix; basement membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NID1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 136 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Bovine (80%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Nidogen-1/Entactin Antibody

• enactin
• Entactin
• Entactin-1
• NID1
• NID-1
• NIDentactin
• nidogen (enactin)
• nidogen 1
• Nidogen1
• Nidogen-1

Background

This gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: NID1
• Uniprot:
  • P14543(Human)