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2570-ND) was serially diluted and captured by Mouse Anti-Human Nidogen‑1/Entactin Monoclonal Antibody (Catalog # MAB2570) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Nidogen‑1/Entactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2570) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
HAF007) or the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox" target="_blank">
2570-ND) was serially diluted and captured by Mouse Anti-Human Nidogen‑1/Entactin Monoclonal Antibody (Catalog # MAB2570) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Nidogen‑1/Entactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2570) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
Entactin encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix. [provided by RefSeq]