NHE3/SLC9A3 Antibody - BSA Free

Western Blot: NHE3/SLC9A3 Antibody [NBP3-00589] - Total protein from human Hek293 and HeLa cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NHE3 (NBP3-00589) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).

Immunocytochemistry/Immunofluorescence: NHE3/SLC9A3 Antibody [NBP3-00589] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-NHE3/SLC9A3 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP3-00589] - Analysis of a FFPE tissue section of human small intestine using 1:200 dilution of NHE3 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This NHE3 antibody generated nuclear staining.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

NHE3/SLC9A3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NHE3/SLC9A3 Antibody - BSA Free (NBP3-00589) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-NHE3/SLC9A3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Partial synthetic peptide made to an internal portion of the human NHE3 protein (between amino acids 600-660) [UniProt P48764]
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC9A3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 5 - 10 ug/ml
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 2 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NHE3/SLC9A3 Antibody - BSA Free

• isoform 3
• MGC126718
• NHE3
• NHE3MGC126720
• SLC9A3
• solute carrier family 9 (sodium/hydrogen exchanger), member 3
• Solute carrier family 9 member 3

Background

Na(+)/H(+) exchanger 3 (NHE3), also known as SLC9A3, plays an important role in neutral Na(+) transport in mammalian epithelial cells. SLC9A3 is acutely up- and down-regulated in response to some G protein-coupled receptors, tyrosine kinase receptors, and protein kinases. It is involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. SLC9A3 was demonstrated to be a modifier of intestinal disease in a murine model of CF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NHE3/SLC9A3 Antibody (NBP3-00589)(1)

Publication using NBP3-00589

• Armando I, Cuevas S, Fan C et al. G Protein-Coupled Receptor 37L1 Modulates Epigenetic Changes in Human Renal Proximal Tubule Cells International journal of molecular sciences 2022-11-21 [PMID: 36430934] (WB, Human)

Bioinformatics

• Gene Symbol: SLC9A3