NGFRAP1/BEX3/NADE Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: NGFRAP1/BEX3/NADE Antibody [NBP1-89987] - Staining of human cell line U-2 OS shows positivity in cytoplasm.

Immunohistochemistry-Paraffin: NGFRAP1/BEX3/NADE Antibody [NBP1-89987] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferus tubules and leydig cells.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NGFRAP1/BEX3/NADE Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: NEEMEQPMQNGEEDRPLGGGEGHQPAGNRRGQARRLAPNFRWAIPNRQINDGMGGDGDDMEIFMEEMREIRRKLRELQLRNCLRILMGELSNHHDHHDEF
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BEX3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  NGFRAP1/BEX3/NADE Protein (NBP1-89987PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for NGFRAP1/BEX3/NADE Antibody - BSA Free

• BEX3
• BEX3p75NTR-associated cell death executor
• Brain-expressed X-linked protein 3
• DXS6984EX-linked 3
• HGR74
• NADE
• nerve growth factor receptor (TNFRSF16) associated protein 1
• Nerve growth factor receptor-associated protein 1
• NGFRAP1
• Ovarian granulosa cell 13.0 kDa protein HGR74
• ovarian granulosa cell protein (13kD)
• protein BEX3

Background

The p75 neurotrophin receptor (p75NTR) is a member of the tumor necrosis receptor superfamily and can mediate cell death and cell survival in response to nerve growth factor (NGF). The p75NTR-associated cell death executor (NADE) mediates apoptosis by interacting with the cell death domain of p75NTR following the binding of NGF by p75NTR. Recent studies have shown that NADE also interacts with second mitochondria-derived activator of caspase (Smac). Coexpression of NADE and Smac promotes TRAIL-induced apoptosis and inhibits XIAP-mediated Smac ubiquitization. It has been suggested that it is this interaction between NADE and Smac that allows apoptosis to proceed. Finally, although initially discovered as an mRNA expressed in ovarian granulosa cells, NADE has been suggested to play a role in the neuronal death seen in epileptic brain damage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BEX3