NGFRAP1/BEX3/NADE Antibody - BSA Free

Western Blot: NGFRAP1/BEX3/NADE Antibody [NBP1-77149] - Human brain cell lysates with NADE antibody at 1 u/mL in the presence (A) or absence (B) of blocking peptide.

Immunohistochemistry: NGFRAP1/BEX3/NADE Antibody - BSA Free [NBP1-77149] - Immunohistochemistry of NGFRAP1/BEX3/NADE in human brain tissue with NGFRAP1/BEX3/NADE antibody at 2 u/mL.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

NGFRAP1/BEX3/NADE Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a peptide corresponding to 14 amino acids near the middle of human NADE. The immunogen is located within the last 50 amino acids of NADE. Amino Acid Squence: EEMREIRRKLRELQ
• Predicted Species: Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BEX3
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 2 ug/ml
  • Immunohistochemistry-Paraffin 2 ug/ml
  • Western Blot 1 ug/ml
• Application Notes: Despite its predicted molecular weight, NGFRAP1/BEX3/NADE migrates at ~23 kDa in SDS-PAGE.
• Control Peptide:

  NGFRAP1/BEX3/NADE Peptide (NBP1-77149PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for NGFRAP1/BEX3/NADE Antibody - BSA Free

• BEX3
• BEX3p75NTR-associated cell death executor
• Brain-expressed X-linked protein 3
• DXS6984EX-linked 3
• HGR74
• NADE
• nerve growth factor receptor (TNFRSF16) associated protein 1
• Nerve growth factor receptor-associated protein 1
• NGFRAP1
• Ovarian granulosa cell 13.0 kDa protein HGR74
• ovarian granulosa cell protein (13kD)
• protein BEX3

Background

The p75 neurotrophin receptor (p75NTR) is a member of the tumor necrosis receptor superfamily and can mediate cell death and cell survival in response to nerve growth factor (NGF). The p75NTR-associated cell death executor (NADE) mediates apoptosis by interacting with the cell death domain of p75NTR following the binding of NGF by p75NTR. Recent studies have shown that NADE also interacts with second mitochondria-derived activator of caspase (Smac). Co-expression of NADE and Smac promotes TRAIL-induced apoptosis and inhibits XIAP-mediated Smac ubiquitization. It has been suggested that it is this interaction between NADE and Smac that allows apoptosis to proceed. Finally, although initially discovered as an mRNA expressed in ovarian granulosa cells, NADE has been suggested to play a role in the neuronal death seen in epileptic brain damage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NGFRAP1/BEX3/NADE Antibody (NBP1-77149)(2)

Publications using NBP1-77149

• Kim By, Park Jy, Cho Kj, Bae Jh Effects of Urban Particulate Matter on the Olfactory System in a Mouse Model American journal of rhinology & allergy 2021-07-08 [PMID: 34236242]
• Kim BY, Park J, Kim E, Kim B Olfactory Ensheathing Cells Mediate Neuroplastic Mechanisms After Olfactory Training in Mouse Model Am J Rhinol Allergy 2019-11-02 [PMID: 31680531]

Bioinformatics

• Gene Symbol: BEX3
• Uniprot:
  • NP_996798(Human)