NFYB Antibody

Images

 
Western Blot: NFYB Antibody [NBP2-19534] - Sample (20 ug ) A: HeLa 12% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: NFYB Antibody [NBP2-19534] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: NFYB Antibody [NBP2-19534] - Paraffin-embedded mouse liver. NFYB antibody [N1C3-2] diluted at 1:500
Immunohistochemistry-Paraffin: NFYB Antibody [NBP2-19534] - Immunohistochemical analysis of paraffin-embedded SAS xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, RM, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NFYB Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human NFYB. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Rat (94%), Porcine (99%), Bovine (98%), Rhesus Macaque (100%), Xenopus (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NFYB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (91%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for NFYB Antibody

  • CAAT box DNA-binding protein subunit B
  • CBF-A
  • HAP3CBF-B
  • NF-YBCCAAT-binding transcription factor subunit A
  • Nuclear transcription factor Y subunit B
  • nuclear transcription factor Y subunit beta
  • nuclear transcription factor Y, beta
  • Transcription factor NF-Y, B subunit

Background

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NFYB
Uniprot