NFYB Antibody - BSA Free

Western Blot: NFYB Antibody [NBP2-19534] - Sample (20 ug ) A: HeLa 12% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: NFYB Antibody [NBP2-19534] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: NFYB Antibody [NBP2-19534] - Paraffin-embedded mouse liver. NFYB antibody [N1C3-2] diluted at 1:500

Immunohistochemistry-Paraffin: NFYB Antibody [NBP2-19534] - Immunohistochemical analysis of paraffin-embedded SAS xenograft, using antibody at 1:500 dilution.

• Reactivity: Hu, Mu, Rt, Po, Bv, RM, Xp
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NFYB Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NFYB Antibody - BSA Free (NBP2-19534) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human NFYB. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Rat (94%), Porcine (99%), Bovine (98%), Rhesus Macaque (100%), Xenopus (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NFYB
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (91%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for NFYB Antibody - BSA Free

• CAAT box DNA-binding protein subunit B
• CBF-A
• HAP3CBF-B
• NF-YBCCAAT-binding transcription factor subunit A
• Nuclear transcription factor Y subunit B
• nuclear transcription factor Y subunit beta
• nuclear transcription factor Y, beta
• Transcription factor NF-Y, B subunit

Background

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: NFYB
• Uniprot:
  • P25208(Human)