NFS1 Antibody - BSA Free

Immunohistochemistry-Paraffin: NFS1 Antibody [NBP2-38737] - Staining of human cerebral cortex, colon, kidney and testis using Anti-NFS1 antibody NBP2-38737 (A) shows similar protein distribution across tissues to independent antibody NBP2-37933 (B).

Western Blot: NFS1 Antibody [NBP2-38737] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4

Immunohistochemistry-Paraffin: NFS1 Antibody [NBP2-38737] - Staining of human cerebral cortex.

Immunohistochemistry-Paraffin: NFS1 Antibody [NBP2-38737] - Staining of human colon.

Immunohistochemistry-Paraffin: NFS1 Antibody [NBP2-38737] - Staining of human kidney.

Immunohistochemistry-Paraffin: NFS1 Antibody [NBP2-38737] - Staining of human testis.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NFS1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: SNNIAIKGVARFYRSRKKHLITTQTEHKCVLDSCRSLEAEGFQVTYLPVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGRICSSRKV
• Predicted Species: Mouse (97%), Rat (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NFS1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  NFS1 Protein (NBP2-38737PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for NFS1 Antibody - BSA Free

• cysteine desulfurase, mitochondrial
• EC 2.8.1.7
• NFS1 nitrogen fixation 1 homolog (S. cerevisiae)
• NifS
• NIFShomolog)
• nitrogen-fixing bacteria S-like protein

Background

Iron-sulfur clusters are required for the function of many cellular enzymes. The protein encoded by this gene supplies inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded protein belongs to the class-V family of pyridoxal phosphate-dependent aminotransferases. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NFS1
• Uniprot:
  • Q9Y697(Human)