NFkB2/NFkB p100 Antibody (291319) [Unconjugated]

Images

 
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human CD40 Ligand/TNFSF5 aa 108-261 (Catalog # 6245-CL) for 4 hours. Gels were loaded with ...read more
NF kappa B2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human NF kappa B2 Monoclonal Antibody (Catalog # MAB28881) at 3 µg/mL for 3 hours at room temperature. ...read more
Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin overnight was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. NF kappa ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ChIP, ICC/IF
Clone
291319
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

NFkB2/NFkB p100 Antibody (291319) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human NF kappa B2
Met1-Asn447
Accession # Q00653
Specificity
Detects human NFkB2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with endogenous mouse NFkB2 in NIH 3T3 cells is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NFKB2
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 3-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NFkB2/NFkB p100 Antibody (291319) [Unconjugated]

  • LYT10
  • NFkB2
  • NF-kB2

Background

Nuclear Factor kappa B2 (NF kappa B2 or NF kappa B p52) is a member of the NF kappa B/Rel family of transcription factors. NF kappa B2 dimerizes with other members of the NF kappa B/Rel family to regulate expression of genes that participate in immune, apoptotic, and oncogenic processes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NFKB2
Entrez
Uniprot