NFkB2 Antibodies

6245-CL) for 4 hours. Gels were loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear extracts (Nuc). For additional reference, lysates of Raji human Burkitt's lymphoma cell line were included. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human NF kappa B2 Monoclonal Antibody (Catalog # MAB28881) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands for NF kappa B2 were detected at approximately 52 kDa and 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> 6245-CL) for 4 hours. Gels were loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear extracts (Nuc). For additional reference, lysates of Raji human Burkitt's lymphoma cell line were included. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human NF kappa B2 Monoclonal Antibody (Catalog # MAB28881) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands for NF kappa B2 were detected at approximately 52 kDa and 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human CD40 Ligand/TNFSF5 aa 108-261 (Catalog # 6245-CL) for 4 hours. Gels were loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear extracts (Nuc). For additional reference, lysates of Raji human Burkitt's lymphoma cell line were included. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human NF kappa B2 Monoclonal Antibody (Catalog # MAB28881) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands for NF kappa B2 were detected at approximately 52 kDa and 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB002) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Mouse IgG Secondary Antibody (Catalog # BAF007). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Thec-mycpromoter was detected by standard PCR." class="big_lightbox"> MAB002) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Mouse IgG Secondary Antibody (Catalog # BAF007). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Thec-mycpromoter was detected by standard PCR." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />