Rat NF-M ELISA Kit (Colorimetric)

ELISA: Rat NF-M ELISA Kit (Colorimetric) [NBP2-78760] - Samples were spiked with high concentrations of Rat NF-M and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat NF-M ELISA Kit (Colorimetric) [NBP2-78760] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids.
• Standard Curve Range: 31.25 - 2000 pg/mL
• Sensitivity: 18.75 pg/mL

Rat NF-M ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat NEF3 in samples. No Significant cross-reactivity or interference between Rat NEF3 and analogues was observed.
• Standard Curve Range: 31.25 - 2000 pg/mL
• Sensitivity: 18.75 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.77%
• Intra-Assay: CV% < 5.21%
• Spike Recovery: 83-101%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: NEFM

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat NF-M ELISA Kit (Colorimetric)

• NEF3
• NEF3neurofilament medium polypeptide
• NEFM
• Neurofilament 3
• neurofilament, medium polypeptide 150kDa
• neurofilament, medium polypeptide
• neurofilament-3 (150 kD medium)
• NFM
• NF-M
• NF-M160 kDa neurofilament protein
• NFMNeurofilament triplet M protein

Background

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-M is the middle or medium neurofilament subunit and runs on SDS-PAGE gels in the range 145-170kDa, with some size variation in across species.

Antibodies to NF-M may be used to identify NF-M in neurons and to study neuronal processes in tissue sections and/or culture. NF-M can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for NF-M ELISA Kit (NBP2-78760). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: NEFM
• Uniprot:
  • P12839(Rat)