Human NF-L ELISA Kit (Colorimetric)

ELISA: Human NF-L ELISA Kit (Colorimetric) [NBP2-81184] - Samples were spiked with high concentrations of Human NF-L and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human NF-L ELISA Kit (Colorimetric) [NBP2-81184] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL

Human NF-L ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human NF-L in samples. No significant cross-reactivity or interference between Human NF-L and analogues was observed.
• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.92%
• Intra-Assay: CV% < 4.94%
• Spike Recovery: 86-106%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: NEFL

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human NF-L ELISA Kit (Colorimetric)

• 68 kDa neurofilament protein
• CMT2E
• NEFL
• neurofilament protein, light chain
• neurofilament subunit NF-L
• Neurofilament triplet L protein
• neurofilament, light polypeptide
• neurofilament-light
• NF68
• NF68FLJ53642
• NFL
• NF-L
• NFLlight polypeptide 68kDa

Background

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-L is the light or low molecular weight polypeptide, and it runs on SDS-PAGE gels at about 68kDa, with some size variation in across species.

Antibodies to NF-L may be used to identify NF-L in neurons and to study neuronal processes in tissue sections and/or culture. NF-L antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for NF-L ELISA Kit (NBP2-81184)(4)

Publications using NBP2-81184

• Shiravi S, Yufa A, Papavasileiou P et al. A Model of Traumatic Brain Injury Oligomerizes Tau in Cortical Organoids and Induces Clinically Relevant Pathologies that Synergize with MAPT Mutation. Journal of Neurotrauma 2025-09-06 [PMID: 40900146]
• Fu Y, He Y, Phan K et al. Increased unsaturated lipids underlie lipid peroxidation in synucleinopathy brain Acta neuropathologica communications 2022-11-14 [PMID: 36376990] (ELISA, Human)
• Magdy R, Eid RA, Fathy W et al. Characteristics and Risk Factors of Persistent Neuropathic Pain in Recovered COVID-19 Patients Pain medicine (Malden, Mass.) 2021-12-21 [PMID: 34931670] (ELISA)
• Shoemaker AR, Jones IE, Jeffris KD Et al. Biofidelic dynamic compression of human cortical spheroids reproduces neurotrauma phenotypes Disease models & mechanisms 2021-11-08 [PMID: 34746950] (ELISA)

FAQs for NF-L ELISA Kit (NBP2-81184). (Showing 1 - 2 of 2 FAQs).

1. I am interested in your elisa kit NBP2-81184 for NF-L test. Can I use cerebrospinal fluid as the semple of my test? is it gonna affect the accuracy of the test with your kit?
   • This kit is validated for biological fluids. However, you should validate your sample type, perhaps by performing spike and recovery.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: NEFL