Mouse NF-L ELISA Kit (Colorimetric)

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ELISA: Mouse NF-L ELISA Kit (Colorimetric) [NBP2-80299] - Standard Reference Curve

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.156-10 ng/mL
Sensitivity
0.094 ng/mL

Order Details

Mouse NF-L ELISA Kit (Colorimetric) Summary

Standard Curve Range
0.156-10 ng/mL
Sensitivity
0.094 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.95%
Intra-Assay
CV% < 4.85%
Spike Recovery
88-105%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
NEFL

Reactivity Notes

No significant cross-reactivity or interference between Mouse NF-L and analogues was observed.

Packaging, Storage & Formulations

Storage
Storage is content dependent.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Certificate of Analysis
  3. Concentrated Biotinylated Detection Ab (100X)
  4. Concentrated Wash Buffer (25X)
  5. Concentrated HRP Conjugate (100X)
  6. Product Description
  7. Plate Sealer
  8. Reference Standard
  9. HRP Conjugate Diluent
  10. Micro ELISA Plate(Dismountable)
  11. Stop Solution
  12. Substrate Reagent
  13. Reference Standard and Sample Diluent

Alternate Names for Mouse NF-L ELISA Kit (Colorimetric)

  • 68 kDa neurofilament protein
  • CMT2E
  • NEFL
  • neurofilament protein, light chain
  • neurofilament subunit NF-L
  • Neurofilament triplet L protein
  • neurofilament, light polypeptide
  • neurofilament-light
  • NF68
  • NF68FLJ53642
  • NFL
  • NF-L
  • NFLlight polypeptide 68kDa

Background

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-L is the light or low molecular weight polypeptide, and it runs on SDS-PAGE gels at about 68kDa, with some size variation in across species.

Antibodies to NF-L may be used to identify NF-L in neurons and to study neuronal processes in tissue sections and/or culture. NF-L antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol NEFL