Mouse NF-L ELISA Kit (Colorimetric)

ELISA: Mouse NF-L ELISA Kit (Colorimetric) [NBP2-80299] - Samples were spiked with high concentrations of Mouse NF-L and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse NF-L ELISA Kit (Colorimetric) [NBP2-80299] - Standard Curve Reference

ELISA: Mouse NF-L ELISA Kit (Colorimetric) [NBP2-80299] - Image from a verified customer review.

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma
• Standard Curve Range: 0.156-10 ng/mL
• Sensitivity: 0.094 ng/mL

Mouse NF-L ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 0.156-10 ng/mL
• Sensitivity: 0.094 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.95%
• Intra-Assay: CV% < 4.85%
• Spike Recovery: 88-105%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: NEFL

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse NF-L ELISA Kit (Colorimetric)

• 68 kDa neurofilament protein
• CMT2E
• NEFL
• neurofilament protein, light chain
• neurofilament subunit NF-L
• Neurofilament triplet L protein
• neurofilament, light polypeptide
• neurofilament-light
• NF68
• NF68FLJ53642
• NFL
• NF-L
• NFLlight polypeptide 68kDa

Background

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-L is the light or low molecular weight polypeptide, and it runs on SDS-PAGE gels at about 68kDa, with some size variation in across species.

Antibodies to NF-L may be used to identify NF-L in neurons and to study neuronal processes in tissue sections and/or culture. NF-L antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for NF-L ELISA Kit (NBP2-80299)(2)

Publications using NBP2-80299

• Berthiaume A, Reda S, Johnston J et al. ATH-1105, a Small Molecule Positive Modulator of HGF/MET, is Neuroprotective and Preserves Motor and Nerve Function in a TDP-43 Mouse Model of ALS Cell Reports Medicine 2023-02-22 (ELISA)
• Jalnapurkar B, Le D, Shainin W et al. A deep-learning-based screening platform in aging-relevant human motor neurons to identify therapeutic compounds for amyotrophic lateral sclerosis bioRxiv 2023-11-22 (ELISA)

Review for NF-L ELISA Kit (NBP2-80299) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Verified Customer 04/17/2024

Summary

• Sample Tested: Blood plasma
• Lot: ZD22F4JJ6187

FAQs for NF-L ELISA Kit (NBP2-80299). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: NEFL