NF-L Antibody

Images

 
Western Blot: NF-L Antibody [NBP1-05219] - Western blots of whole protein extracts of rat spinal cord (Lanes 1), brain stem (Lanes 2), cerebellum (Lanes 3) and cerebral cortex (Lanes 4). Neurofilaments are concentrated ...read more
Immunocytochemistry/ Immunofluorescence: NF-L Antibody [NBP1-05219] - View of mixed neuron/glial cultures stained with NBP1-05219 (red) and rabbit antibody to phosphorylated NF-H (NB300-135, green). The NF-L protein is ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated

Order Details

NF-L Antibody Summary

Immunogen
Neurofilament Light protein prepared from bovine spinal cord [UniProt# P02548]
Marker
Neuronal Marker
Isotype
IgY
Clonality
Polyclonal
Host
Chicken
Gene
NEFL
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Immunocytochemistry/Immunofluorescence 1:5000
Application Notes
This 68kDa Neurofilament Light antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at ~68 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
5mM Sodium Azide
Purity
Ammonium sulfate precipitation

Alternate Names for NF-L Antibody

  • 68 kDa neurofilament protein
  • CMT2E
  • NEFL
  • neurofilament protein, light chain
  • neurofilament subunit NF-L
  • Neurofilament triplet L protein
  • neurofilament, light polypeptide
  • neurofilament-light
  • NF68
  • NF68FLJ53642
  • NFL
  • NF-L
  • NFLlight polypeptide 68kDa

Background

Neuronal intermediate filaments or neurofilaments are found specifically in neurons where they play a key role in maintaining neuronal morphology as well as in regenerating myelinated axons. Neurofilaments contain three subunits: a light polypeptide (NEFL/NFL), a medium polypeptide (NEFM/NFM), and a heavy polypeptide (NEFH/NFH), with molecular weights of 68, 160, and 212 kD respectively. 68kD Neurofilament Light (NEFL) is the most abundant of three neurofilament proteins and, like other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments. NEFL forms the backbone of neurofilament and perturbations in its expression leads to motor neuron diseases, such as Charcot-Marie-Tooth disease, type 2E (CMT2E). In addition to its influence on the nervous system, NEFL has been shown to act as a tumor suppressor and has been linked to carcinogenesis/metastasis of several malignancues including breast, prostate, lung, colon, and bladder cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NEFL
Entrez
Uniprot