NF-L Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: NF-L Antibody [NBP1-05219] - View of mixed neuron/glial cultures stained with NBP1-05219 (red) and rabbit antibody to phosphorylated NF-H (NB300-135, green). The NF-L protein is ...read more
NF-L Antibody [NBP1-05219] - Immunofluorescent analysis of rat cerebellum section stained with chicken pAb to NF-L, NBP1-05219, dilution 1:2,000 in green, and costained with mouse mAb to FOX3/NeuN, dilution 1:5,000 in ...read more
NF-L Antibody [NBP1-05219] - Western blot analysis of tissue lysates probed with chicken pAb to NF-L, NBP1-05219, dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated

Order Details

NF-L Antibody Summary

Immunogen
Recombinant human NF-L protein
Marker
Neuronal Marker
Isotype
IgY
Clonality
Polyclonal
Host
Chicken
Gene
NEFL
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000
  • Immunocytochemistry/Immunofluorescence 1:2000
  • Immunohistochemistry 1:2000
Application Notes
This 68kDa Neurofilament Light antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at ~68 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
5mM Sodium Azide
Purity
Ammonium sulfate precipitation

Alternate Names for NF-L Antibody

  • 68 kDa neurofilament protein
  • CMT2E
  • NEFL
  • neurofilament protein, light chain
  • neurofilament subunit NF-L
  • Neurofilament triplet L protein
  • neurofilament, light polypeptide
  • neurofilament-light
  • NF68
  • NF68FLJ53642
  • NFL
  • NF-L
  • NFLlight polypeptide 68kDa

Background

Neuronal intermediate filaments or neurofilaments are found specifically in neurons where they play a key role in maintaining neuronal morphology as well as in regenerating myelinated axons. Neurofilaments contain three subunits: a light polypeptide (NEFL/NFL), a medium polypeptide (NEFM/NFM), and a heavy polypeptide (NEFH/NFH), with molecular weights of 68, 160, and 212 kD respectively. 68kD Neurofilament Light (NEFL) is the most abundant of three neurofilament proteins and, like other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments. NEFL forms the backbone of neurofilament and perturbations in its expression leads to motor neuron diseases, such as Charcot-Marie-Tooth disease, type 2E (CMT2E). In addition to its influence on the nervous system, NEFL has been shown to act as a tumor suppressor and has been linked to carcinogenesis/metastasis of several malignancues including breast, prostate, lung, colon, and bladder cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB300-133
Species: Hu, Mu, Rt, Po, Bv, Ch, Eq
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NB300-217
Species: Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe
Applications: WB, ICC/IF, IHC, ELISA(Cap)
AF3235
Species: Hu, Mu, Rt
Applications: WB, IHC
NB300-141
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NB300-223
Species: Hu, Mu, Rt, Po, Bv, Ch, Eq
Applications: WB, ICC/IF, ICC
NBP2-42388
Species: Hu
Applications: WB, IHC, IHC-P, KD
NB100-1607
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB300-213
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IF
NBP2-25162
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF, IHC
NB300-140
Species: Hu, Mu, Rt, Bv
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP2-24915
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC-P
NB120-15200
Species: Hu, Mu, Sh
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-57493
Species: Hu, Mu
Applications: WB, IP
NB300-137
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC
NBP2-53384
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC-P
NB110-58870
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC
NBP1-05219
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ICC/IF, IHC

Publications for NF-L Antibody (NBP1-05219) (0)

There are no publications for NF-L Antibody (NBP1-05219).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for NF-L Antibody (NBP1-05219) (0)

There are no reviews for NF-L Antibody (NBP1-05219). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NF-L Antibody (NBP1-05219) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional NF-L Products

Bioinformatics Tool for NF-L Antibody (NBP1-05219)

Discover related pathways, diseases and genes to NF-L Antibody (NBP1-05219). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for NF-L Antibody (NBP1-05219)

Discover more about diseases related to NF-L Antibody (NBP1-05219).
 

Pathways for NF-L Antibody (NBP1-05219)

View related products by pathway.

PTMs for NF-L Antibody (NBP1-05219)

Learn more about PTMs related to NF-L Antibody (NBP1-05219).
 

Research Areas for NF-L Antibody (NBP1-05219)

Find related products by research area.

Blogs on NF-L

There are no specific blogs for NF-L, but you can read our latest blog posts.

Customers Who Bought This Also Bought

GM-CSF [Unconjugated]
215-GM-010/CF

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our NF-L Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol NEFL
Entrez
Uniprot