![Western Blot: NF-L Antibody [NBP1-05219] - Analysis of tissue lysates probed with chicken pAb to NF-L, NBP1-05219, dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord and [6] cow spinal cord. Strong bands at ~68kDa corresponds to NF-L proteins which are known to have slightly different apparent SDS-PAGE molecular weights across species boundaries.](http://images.novusbio.com/fullsize/NF-L-Antibody-NBP1-05219-img0006.jpg "Western Blot: NF-L Antibody [NBP1-05219] - Analysis of tissue lysates probed with chicken pAb to NF-L, NBP1-05219, dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord and [6] cow spinal cord. Strong bands at ~68kDa corresponds to NF-L proteins which are known to have slightly different apparent SDS-PAGE molecular weights across species boundaries.")
| Reactivity | Hu, Mu, Rt, Po, BvSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IHC |
| Clonality | Polyclonal |
| Host | Chicken |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Chicken NF-L Antibody - BSA Free (NBP1-05219) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Recombinant human NF-L protein |
| Marker | Neuronal Marker |
| Isotype | IgY |
| Clonality | Polyclonal |
| Host | Chicken |
| Gene | NEFL |
| Purity | IgY purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This 68kDa Neurofilament Light antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at ~68 kDa. |
| Theoretical MW | 68 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Supplied as concentrated total IgY preparation from egg yolk. Exact concentration of target specific IgY is not quantifiable as the preparation contains both immune IgY specific for the target and also irrelevant, non-immune IgY. |
| Preservative | 0.02% Sodium Azide |
| Purity | IgY purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for NF-L Antibody (NBP1-05219)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: NF-L Antibody [NBP1-05219] - View of mixed neuron/glial cultures stained with NBP1-05219 (red) and rabbit antibody to phosphorylated NF-H (NB300-135, green). The NF-L protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells. In contrast the phosphorylated NF-H has a much more restricted expression pattern, being found only in developed axonal neurofilaments. Since both proteins are found in neurofilaments, the red and green patterns overlap, so that neurofilaments containing NF-L and phosphorylated NF-H appear yellowish. In contrast neurofilaments containing only NF-L appear red.](http://images.novusbio.com/fullsize/NF-L-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-05219-img0004.jpg "Immunocytochemistry/Immunofluorescence: NF-L Antibody [NBP1-05219] - View of mixed neuron/glial cultures stained with NBP1-05219 (red) and rabbit antibody to phosphorylated NF-H (NB300-135, green). The NF-L protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells. In contrast the phosphorylated NF-H has a much more restricted expression pattern, being found only in developed axonal neurofilaments. Since both proteins are found in neurofilaments, the red and green patterns overlap, so that neurofilaments containing NF-L and phosphorylated NF-H appear yellowish. In contrast neurofilaments containing only NF-L appear red.")
![Immunohistochemistry Free-Floating: NF-L Antibody [NBP1-05219] - Staining of rat cerebellum section stained with chicken pAb to NF-L, NBP1-05219, dilution 1:2,000 in green, and costained with mouse mAb to FOX3/NeuN, dilution 1:5,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. NBP1-05219 antibody labels perikarya and processes of neuronal cells, particularly strongly the axons of basket cells, while the FOX3/NeuN antibody stains the nuclei and proximal cytoplasm of neurons.](http://images.novusbio.com/fullsize/NF-L-Antibody-NBP1-05219-img0005.jpg "Immunohistochemistry Free-Floating: NF-L Antibody [NBP1-05219] - Staining of rat cerebellum section stained with chicken pAb to NF-L, NBP1-05219, dilution 1:2,000 in green, and costained with mouse mAb to FOX3/NeuN, dilution 1:5,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. NBP1-05219 antibody labels perikarya and processes of neuronal cells, particularly strongly the axons of basket cells, while the FOX3/NeuN antibody stains the nuclei and proximal cytoplasm of neurons.")
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