NF-L Antibody (DA2)

Western Blot: NF-L Antibody (DA2) [NBP2-31202] - Analysis of whole tissue lysates using mouse mAb to NF-L, NBP2-31202, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at 68-70kDa corresponds to the NF-L protein.

Immunocytochemistry/Immunofluorescence: NF-L Antibody (DA2) [NBP2-31202] - Analysis of rat frontal cortex section stained with mouse mAb to NF-L, NBP2-31202, dilution 1:500 in red, and costained with chicken pAb to GFAP, dilution 1:5,000 in green. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. NBP2-31202 antibody labels cell bodies and processes of pyramidal neurons, as well as dendrites and axons of other neuronal cells, while the GFAP antibody stains the network of glial cells.

Product Discontinued

NF-L Antibody (DA2) Summary

• Immunogen: Enzymatically dephosphorylated full length pig NF-L protein.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NEFL
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Immunohistochemistry 1:1000
  • Western Blot 1:5000
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50% PBS, 50% Glycerol
• Preservative: 0.035% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for NF-L Antibody (DA2)

• 68 kDa neurofilament protein
• CMT2E
• NEFL
• neurofilament protein, light chain
• neurofilament subunit NF-L
• Neurofilament triplet L protein
• neurofilament, light polypeptide
• neurofilament-light
• NF68
• NF68FLJ53642
• NFL
• NF-L
• NFLlight polypeptide 68kDa

Background

Neurofilaments are members of the intermediate filament protein family and are neuron-specific. There are three major proteins, NF-H, NF-L and NF-M. Accumulation of neurofilaments is observed during the progression of numerous neurological diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NEFL
• Entrez:
  • 4747(Human)
• Uniprot:
  • O36635(Human)