NF-H Antibody

Product Discontinued

NF-H Antibody Summary

• Immunogen: Rabbits were immunized with purified bovine neurofilament NF-H.
• Marker: Neuronal Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NEFH
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Western Blot 1:1000-1:2500

Reactivity Notes

Most Mammals

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 1 mg/ml BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for NF-H Antibody

• 200 kDa neurofilament protein
• KIAA0845
• NEFH
• Neurofilament Heavy (200kDa)
• neurofilament heavy polypeptide
• Neurofilament triplet H protein
• neurofilament, heavy polypeptide 200kDa
• neurofilament, heavy polypeptide
• NF200
• NFH
• NF-H

Background

Neurofilaments are members of the intermediate filament protein family and are neuron-specific. There are three major proteins, NF-H, NF-L and NF-M. Accumulation of neurofilaments is observed during the progression of numerous neurological diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NF-H Antibody (NBP2-25030)(1)

Publication using NBP2-25030

• Shaw G, Yang C, Ellis R et al. Hyperphosphorylated neurofilament NF-H is a serum biomarker of axonal injury. Biochem Biophys Res Commun. 2005-11-04 [PMID: 16176808] (ICC/IF, WB)
  
  Details:
  ELISA Fig 2 (standard curve with recombinant NF-H protein), Figs 3, 4 (serum from rats with spinal cord injury; IF/ICC (primary neuron/glial cultures from newborn rats), Fig 1B; WB: (rat spinal cord), Fig 1A.

Bioinformatics

• Gene Symbol: NEFH