Neuropilin-1 Antibody (130603)

• Reactivity: Rt
• Applications: Flow, B/N, CyTOF-ready
• Clone: 130603
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Neuropilin-1 Antibody (130603) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat Neuropilin‑1
  Phe22-Asp854 (Lys811Arg, Pro812-Gly828 del), predicted
  Accession # Q9QWJ9
• Specificity: Detects rat Neuropilin-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant rat Neuropilin-2 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NRP1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction 5 ug/mL
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 6,054,293, 6,623,738, and other U.S. and international patents pending.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neuropilin-1 Antibody (130603)

• BDCA4
• BDCA-4
• CD304 antigen
• CD304
• DKFZp686A03134
• DKFZp781F1414
• neuropilin 1
• Neuropilin1
• Neuropilin-1
• NP1
• NRP
• NRP1
• transmembrane receptor
• Vascular endothelial cell growth factor 165 receptor
• VEGF165R

Background

Neuropilin-1 is a type I transmembrane protein that is expressed in the developing nervous system and by endothelial and tumor cells. Neuropilin-1 binds members of the class III secreted semaphorin subfamily as well as some isoforms of VEGF family proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neuropilin-1 Antibody (MAB566)(3)

Publications using MAB566

• Hongjie Pan, Robin E. Bachelder Autocrine Semaphorin3A stimulates eukaryotic initiation factor 4E-dependent RhoA translation in breast tumor cells Experimental Cell Research 2010-10-15 [PMID: 20655307]
• Wanami LS, Chen HY, Peiro S, Garcia de Herreros A, Bachelder RE Vascular endothelial growth factor-A stimulates Snail expression in breast tumor cells: implications for tumor progression. Exp. Cell Res., 2008-05-17;314(13):2448-53. 2008-05-17 [PMID: 18554584] (Neutralization, Human)
• Hacquard-Bouder C, Chimenti MS, Giquel B, Donnadieu E, Fert I, Schmitt A, Andre C, Breban M Alteration of antigen-independent immunologic synapse formation between dendritic cells from HLA-B27-transgenic rats and CD4+ T cells: selective impairment of costimulatory molecule engagement by mature HLA-B27. Arthritis Rheum., 2007-05-01;56(5):1478-89. 2007-05-01 [PMID: 17469106] (Flow Cytometry, Rat)

FAQs for Neuropilin-1 Antibody (MAB566). (Showing 1 - 2 of 2 FAQs).

1. If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?
   • If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.
2. What research areas can Neuropilin-1 antibodies be used in?
   • Neuropilin-1 products can be applied in the following research areas: Adaptive Immunity, Angiogenesis, Breast Cancer, Cancer, Cardiovascular Biology, Neuroscience, and Virology Bacteria and Parasites.

Bioinformatics

• Gene Symbol: NRP1
• Uniprot:
  • Q9QWJ9()