Neurogenin-3 Antibody [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 532

Neurogenin-3 Antibody [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant human Neurogenin-3
  Met1-Leu214
  Accession # Q9Y4Z2
• Specificity: Detects human Neurogenin-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse Neurogenin-3 is observed, and less than 1% cross-reactivity with recombinant human Neurogenin-1 is observe
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neurogenin-3 Antibody [Alexa Fluor® 532]

• Atoh5
• BHLHA7
• bHLHa7NGN-3
• Class A basic helix-loop-helix protein 7
• Math4B
• NEUROG3
• neurogenin 3
• Neurogenin3
• Neurogenin-3
• NGN3
• Protein atonal homolog 5
• Relax

Background

Neurogenin-3 (NGN3; also Protein atonal homolog 5) is a 23 kDa, developmentally expressed nuclear protein that belongs to a family of atonal-related basic helix‑loop‑helix (bHLH) transcription factors. bHLH proteins typically form dimers when binding to DNA. NGN3 expression is required for the lineage differentiation of all endocrine cell types in the pancreas, including the formation of islet beta  cells. NGN3 is also neurogenic, controlling the number of dendrites generated by hippocampal neurons. Human NGN3 is 214 amino acids (aa) in length. It contains a DNA binding region (aa 84‑95) plus an HLH motif (aa 96‑136). Over aa 3‑214, human NGN3 shares 76% and 84% aa identity with mouse and canine NGN3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.