Neurofascin Antibody

Western Blot: Neurofascin Antibody [NBP1-78794] - (0.5ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Neurofascin Antibody Summary

• Description: Novus Biologicals Goat Neurofascin Antibody (NBP1-78794) is a polyclonal antibody validated for use in WB and ELISA. Anti-Neurofascin Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-NFSPNQTKFTVQRT corresponding to internal region according to NP_001153803.1, NP_001153804.1, NP_055905.2.
• Specificity: This antibody is expected to recognize reported isoforms 1, 2, 3 and 4 (NP_001005388.2;,NP_001153803.1, NP_001153804.1and NP_055905.2 resp) .
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NFASC
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.5 - 2 ug/ml
• Application Notes: WB: Approx. 170 kDa band observed in human lung and rat brain lysates is reported in scientific literature (PMID: 19666467). Preliminary testing was unsuccessful on Mouse and Rat Brain for this particular batch.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Neurofascin Antibody

• DKFZp686P2250
• KIAA0756FLJ46866
• neurofascin homolog (chicken)
• neurofascin homolog
• Neurofascin
• NF
• NFASC
• NRCAML

Background

NFASC (Neurofascin) is involved in cell adhesion, axon subcellular targeting, synapse formation, regulating functions of the brain, and axonal guidance. NFASC is known to have interactions with SDCBP, ABL1, ANK1, CRK and DCX. NFASC has been studied in relation to multiple sclerosis, carcinoma, neuronitis and Parkinson's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neurofascin Antibody (NBP1-78794)(2)

Publications using NBP1-78794

• Liu H, Focia PJ, He X. Homophilic adhesion mechanism of neurofascin, a member of the L1 family of neural cell adhesion molecules. J Biol Chem 2011-01-07 [PMID: 21047790]
• Kirschbaum K, Kriebel M, Kranz EU et al. Analysis of non-canonical fibroblast growth factor receptor 1 (FGFR1) interaction reveals regulatory and activating domains of neurofascin. J Biol Chem 2009-10-01 [PMID: 19666467]

Bioinformatics

• Gene Symbol: NFASC
• Entrez:
  • 23114(Human)
  • 269116(Mouse)
  • 116690(Rat)
• Uniprot:
  • NP_001005388.2(Human)