Recombinant Human NEU-1/Sialidase-1 His Protein

SDS-Page: Recombinant Human NEU-1/Sialidase-1 Protein [NBP2-23471]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.25 mg/ml

Recombinant Human NEU-1/Sialidase-1 His Protein Summary

• Description: A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 48-415 of Human NEU-1/Sialidase-1
  
  

  Source: E.coli

  Amino Acid Sequence: GSSHHHHHH SSGLVPRGSH MGSHMENDFG LVQPLVTMEQ LLWVSGRQIG SVDTFRIPLI TATPRGTLLA FAEARKMSSS DEGAKFIALR RSMDQGSTWS PTAFIVNDGD VPDGLNLGAV VSDVETGVVF LFYSLCAHKA GCQVASTMLV WSKDDGVSWS TPRNLSLDIG TEVFAPGPGS GIQKQREPRK GRLIVCGHGT LERDGVFCLL SDDHGASWRY GSGVSGIPYG QPKQENDFNP DECQPYELPD GSVVINARNQ NNYHCHCRIV LRSYDACDTL RPRDVTFDPE LVDPVVAAGA VVTSSGIVFF SNPAHPEFRV NLTLRWSFSN GTSWRKETVQ LWPGPSGYSS LATLEGSMDG EEQAPQLYVL YEKGRNHYTE SISVAKISVY GTL

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: NEU1
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 42.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 10% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human NEU-1/Sialidase-1 His Protein

• Acetylneuraminyl hydrolase
• EC 3.2.1.18
• exo-alpha-sialidase
• FLJ93471
• G9 sialidase
• Lysosomal sialidase
• N-acetyl-alpha-neuraminidase 1
• NANH
• NEU
• NEU1
• NEU-1
• SIAL1
• sialidase 1 (lysosomal sialidase)
• Sialidase-1

Background

NEU1 is a lysosomal enzyme that cleaves terminal sialic acid residues from substrates such as glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A. Mutations in this gene can lead to sialidosis, a lysosomal storage disease that can be type 1 (cherry red -myoclonus syndrome or normosomatic type), which is late-onset, or type 2 (the dysmorphic type), which occurs at an earlier age with increased severity. Recombinant human NEU1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for NEU-1/Sialidase-1 Recombinant Protein (NBP2-23471)(1)

Publication using NBP2-23471

• Klein J, Baker N, Foil D, et al. Using Bibliometric Analysis and Machine Learning to Identify Compounds Binding to Sialidase-1 ACS Omega 2021-02-02 [PMID: 33553934]

Bioinformatics

• Gene Symbol: NEU1