Netrin-2 Antibody [Alexa Fluor® 700]

• Reactivity: Ch
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 700

Netrin-2 Antibody [Alexa Fluor® 700] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant chicken Netrin-2
  Ala16-Pro581
  Accession # Q90923
• Specificity: Detects chicken Netrin‑2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant chicken Netrin-1 is observed and less than 1% cross-reactivity with recombinant human Netrin-4, recombinant mouse (rm)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Netrin-2 Antibody [Alexa Fluor® 700]

• Netrin2
• Netrin-2
• NTN2L

Background

Netrins are laminin-like axon guidance molecules that are conserved among C. elegans, Drosophila, and vertebrates. The chicken Netrin-2 cDNA encodes a precursor protein that has structural similarity to the N-terminus of B2 or the gamma -chain of laminin. The mature Netrin-2 protein has 581 amino acid (aa) which contains a 245 aa domain VI, a 169 aa domain V with three cysteine/glycine rich EGF repeats and a unique 152 aa domain C that enriches in basic aa residues and contains an RGD motif. Chicken Netrin-2 shares 72% aa identity with chicken Netrin-1, 51% aa identity with C. elegans UNC-6 protein and 57% aa identity with human NTN2L (Netrin‑2‑like). Although only two chicken netrins are known, the number of known mammalian netrins is growing. Mouse Netrin-3 and Netrin-4 were recently reported. Netrin-2 was expressed in the chick brain and spinal cord from E6 through to the adult. In addition, expression of Netrin-2 was observed at various stages in several mesodermally and endodermally derived tissues, including lung, gut, ovary, testes and spleen. Netrins act to both attract and repel the growing axons of a broad range of neuronal cell types during development. These actions are mediated by specific receptor complexes containing either the DCC (colorectal carcinoma) or neogenin, in the case of attractant, or UNC-5-related proteins, in the case of repellent.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.