Netrin-1 Antibody

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Immunocytochemistry/ Immunofluorescence: Netrin-1 Antibody [NB100-1605] - Neurosphere (organoid) cultures of e13 mouse brain were cultured for 2 weeks, and then paraformaldehyde (2%) fixed. After extensive washing, the ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

Netrin-1 Antibody Summary

Immunogen
Chickens were immunized with a synthetic peptide-keyhole limpet hemocyanin (KLH) conjugate. This synthetic peptide corresponded to Netrin-1, but was shared between the human (O_095631, NCBI) and mouse (O_09118, NCBI) sequences.
Marker
Neuron Cell Marker
Isotype
IgY
Clonality
Polyclonal
Host
Chicken
Gene
NTN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:1000-1:2000
  • Immunohistochemistry 1:1000-1:2000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:1000-1:2000
  • Western Blot
Application Notes
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 26482371). Use in Western Blot reported in scientific literature (PMID 20628609).
Theoretical MW
67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-1605 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
10mM PBS (0.9% isotonic, w/v, pH 7.2)
Preservative
0.02% Sodium Azide
Concentration
0.1 mg/ml
Purity
Immunogen affinity purified

Notes

Chicken products cannot be exported to Canada.

Purification Notes
After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 100 ug/ml, and the preparation filter-sterilized.

Storage Notes
Store at 4C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

Alternate Names for Netrin-1 Antibody

  • netrin 1
  • Netrin1
  • Netrin-1
  • NTN1
  • NTN1Lnetrin 1, mouse, homolog of

Background

Semaphorins, neuropilins and netrins are among a number of molecules and their receptors that regulate the developing nervous system to guide the development of neural circuits.1 Although first identified as axon guidance cues,2,3 it is now apparent that many of these same factors are not limited to the guidance of growing axons, but have roles in a range of processes from the guidance of cell migration to the regulation of the immune response, angiogenesis, lung branching morphogenesis, nervous system regeneration, and cancer.4-9 The semaphorins make up the largest family of axon guidance cues. They are characterized by the presence of an approximately 500 amino acid N-terminal semaphorin (Sema) domain. Semaphorins function mainly as chemorepellents that direct axons away from tissues.3 Semaphorin 3A (Sema3A) has been shown to be repellent to cortical axons and to inhibit axon branching.10 The transmembrane protein semaphorin 6A has been shown to repel embryonic sympathetic axons.11 The actions of the various semaphorins are not always similar. Semaphorin 3A has been found to inhibit tumor development whereas semaphorin 6A may contribute to tumor progression.9 Neuropilins are the ligand binding moieties in the class 3 Semaphorin receptor complexes that subsequently activate signaling through associated plexins. Two types have been identified so far: Neuropilin-1 (Npn-1) and Neuropilin-2 (Npn-2) receptors. At the amino acid sequence level, Npn-1 and Npn-2 share 44% identity. Npn-1 and Npn-2 show different expression patterns in developing neurons of the central and peripheral nervous systems, and show different binding specificities for different members of he semaphorin family. Both also function as receptors for some forms of vascular endothelial growth factor (VEGF).12 Netrins are a family of laminin-related small proteins that are involved in axon guidance and eurite outgrowth. Netrin-1 has been shown to attract cortical growth cones and promote axon branching.10 Netrin-4 (first named b-netrin) was found to promote neurite elongation from olfactory bulb explants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Netrin-1 Antibody (NB100-1605)(7)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Bovine, Porcine, Sheep.

We have publications tested in 5 applications: ICC/IF, IF/IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(2)
IF/IHC
(1)
IHC-Fr
(1)
IHC-P
(1)
WB
(2)
All Applications
Filter By Species
Human
(1)
Mouse
(3)
Rat
(2)
Bovine
(1)
Porcine
(1)
Sheep
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB100-1605 Applications Species
Cuesta, S, Restrepo-Lozano, J M Et al. DCC-related developmental effects of abused- versus therapeutic-like amphetamine doses in adolescence. Addict Biol 2020-07-01 [PMID: 31192517] (ICC/IF, Sheep, Bovine, Porcine, Mouse) ICC/IF Sheep, Bovine, Porcine, Mouse
Salameh S, Nouel D, Flores C, Hoops D. An optimized immunohistochemistry protocol for detecting the guidance cue Netrin-1 in neural tissue. MethodsX 2018-12-13 [PMID: 29276694] (IHC-P, Mouse) IHC-P Mouse
Reynolds LM, Pokinko M, Torres-Berrio A et al. DCC Receptors Drive Prefrontal Cortex Maturation by Determining Dopamine Axon Targeting in Adolescence. Biol. Psychiatry 2018-01-15 [PMID: 28720317] (WB, Mouse) WB Mouse
Wu CH, Yuan XC, Gao F et al. Netrin-1 Contributes to Myelinated Afferent Fiber Sprouting and Neuropathic Pain. Mol. Neurobiol. 2015-10-19 [PMID: 26482371] (IHC-Fr, Rat) IHC-Fr Rat
Manitt C, Mimee A, Eng C et al. The Netrin Receptor DCC Is Required in the Pubertal Organization of Mesocortical Dopamine Circuitry. J Neurosci;31(23):8381-94. 2011-06-08 [PMID: 21653843]
Tallman MS, Kim HT, Montesinos P et al. Does microgranular variant morphology of acute promyelocytic leukemia independently predict a less favorable outcome compared with classical M3 APL? A joint study of the North American Intergroup and the PETHEMA Group. Blood 2010-12-16 [PMID: 20858857] (Human) Human
Manitt C, Labelle-Dumais C, Eng C et al. Peri-pubertal emergence of UNC-5 homologue expression by dopamine neurons in rodents. PLoS One 2010-07-08 [PMID: 20628609] (ICC/IF, IF/IHC, WB, Rat) ICC/IF, IF/IHC, WB Rat

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Product General Protocols

View specific protocols for Netrin-1 Antibody (NB100-1605): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NTN1
Entrez
Uniprot